一次法咬合重建对大鼠咬肌组织损伤相关蛋白表达的影响  

The effects of one-visit occlusal rehabilitation on the expression of injury-sensitive proteins in rat's masseter muscle

作　　者：崔洁[1] 于世宾[2] 何惠明[1] 张春宝[3] 

机构地区：[1]第四军医大学口腔医学院军事口腔医学国家重点实验室修复科,陕西西安710032 [2]第四军医大学口腔医学院军事口腔医学国家重点实验室颞下颌关节病科,陕西西安710032 [3]第四军医大学口腔医学院军事口腔医学国家重点实验室修复工艺科,陕西西安710032

出　　处：《牙体牙髓牙周病学杂志》2015年第1期20-23,27,共5页Chinese Journal of Conservative Dentistry

基　　金：全军后勤科研计划面上项目(CWS12J101)

摘　　要：目的:探讨一次法咬合重建后大鼠咬肌损伤相关结蛋白(desmin)和骨骼肌肌钙蛋白抑制亚基(s Tn I)含量的变化。方法:取8周龄健康雄性SD大鼠60只,随机分为空白对照组(C组)、操作对照组(S组)和一次法咬合重建组(T组)(n=20)。T组采用人工逐次磨除法将各大鼠上颌后牙牙冠磨低约1.2 mm,6周后口外制作咬合板并将其粘固于各大鼠的上颌后牙上;S组麻醉后模拟T组操作过程,无实际操作;C组不作任何处理。咬合重建后3、7、14、28 d处死大鼠(n=5),取其双侧咬肌组织并采用western blot方法检测咬肌细胞内desmin和s Tn I的表达。结果:1 S组与C组相比desmin、s Tn I表达无明显差异(P>0.05);2 T组于咬合重建后3 d时desmin、s Tn I开始降低(P<0.05),7 d时降至最低,14 d后逐渐增加,28 d时恢复正常;28 d时desmin、s Tn I的表达水平与C组、S组相比P>0.05,其他各时间点的desmin、s Tn I表达水平均低于C组和S组(P<0.05)。结论:一次法咬合重建可造成咬肌细胞内desmin和s Tn I发生不同程度的降解,但这种变化是可逆的。AIM：To investigate the effects of one-visit occlusal rehabilitation（OR）on the expression of desmin and skeletal troponin I （sTnI）in rat＆#39;s masseter muscle.METHODS：60 healthy 8-week-old male SD rats were randomly divided into control group （C）,sham operation group （S）and one-visit OR group （T）（n=20）.In T group,the crown height of the upper molars of the rats were shortened by 1 .2 mm mechanically and gradually,6 weeks later,the occlusal splints were made and adhered to the molar surface by resin bonding.In S group the sham operation was used,the controls were without any operation.5 rats in every group were sacrificed at 3 d,7 d,14 d,and 28 d af-ter operation respectively.The deep masseter muscles were excised,western blot method was used to detect the expres-sion of desmin and sTnI.RESULTS：① The expression of desmin and sTnI showed no significant difference between group C and S（P〉0.05）.②Desmin and sTnI in the masseter muscle in group T decreased 3 d after OR（P〈0.05）, decreased to the lowest level 7 d after OR（P〈0.05 ）,then began to increase gradually and reached the normal level 28 d after the OR（P〉0.05）.CONCLUSION：One-visit occlusal rehabilitation can cause the degradation of desmin and sTnI in masseter muscle.However,the damage is reversible.

关 键 词：一次法咬合重建 咬肌 结蛋白(desmin) 骨骼肌肌钙蛋白抑制亚基(s Tn I) 

分 类 号：R780.2[医药卫生—口腔医学]