生长抑制和DNA损伤基因45a在人不同组织类型涎腺肿瘤中的表达  

Expression of Growth Arrest and DNA Damage-Inducible45a Gene in Different Types of Human Oral Salivary Gland Tumors

作　　者：郭淑荣[1] 董刚[2] 李涛[2] 郑建金[2] 齐方梅[2] 徐燕[1] 卢恕东 

机构地区：[1]青岛大学附属青岛市市立医院口腔科,山东省青岛市266011 [2]青岛市市立医院口腔医学中心,山东省青岛市266011

出　　处：《医学分子生物学杂志》2015年第1期7-11,共5页Journal of Medical Molecular Biology

基　　金：青岛市科技局课题（No.KJZD-13-15-NSH,11-2-3-2-（7）-nsh）,青岛市卫生局课题（No.2012.WSZD051）,山东省卫计委课题（Nn2014-WS2018）

摘　　要：目的探讨生长抑制和DNA损伤基因45a（Gadd45a）mRNA在涎腺正常组织及其良、恶性肿瘤组织中的表达情况及其临床病理意义。方法应用逆转录聚合酶链反应（RT-PCR）和实时荧光定量PCR（real．timePCR）方法检测30例正常涎腺组织和涎腺良、恶性肿瘤中Gadd45amRNA的表达量。结果与瘤旁正常腮腺组织相比，Gadd45amRNA在恶性肿瘤中的表达量降低；Gadd45amRNA在多形性腺瘤中的表达量，在基底细胞腺瘤中的表达量亦降低，Gadd45amRNA在腺淋巴瘤中的表达量未见明显变化。Gadd45amRNA在恶性肿瘤中表达较良性肿瘤（多形性腺瘤和基底细胞腺瘤）降低（P〈0．05）。结论提示Gadd45a基因可能在涎腺肿瘤的发生发展中具有重要作用，Gadd45amRNA在恶性涎腺肿瘤中低表达可能诱导了肿瘤细胞的恶性增殖。Objective To investigate the expression of Growth arrest and DNA damage-induc- ible 45a （ Gadd45a ） mRNA in normal, benign, and malignant tissues of salivary gland and its pathological significance. Methods 30 cases of salivary gland tumors from human and the same number of salivary gland tissues adjacent to the tumors were used in this study. The changes of Gadd45a mRNA expression level were detected by reverse transcription-polymerase chain reaction （RT-PCR） followed by Real-time PCR. Results Compared to adjacent salivary gland tissues, the Gadd45a mRNA expression in malignant tumors were decreased significantly, the C, adclA5a mRNA expression in pleomorphic adenoma and basal cell adenoma were decreased too; But the Gadd45a mRNA expression in warthin tumors were no significant changes. The C, add45a mRNA expression were lower in malignant salivary gland tumors than that in benign （pleomorphic adenoma and basal cell adenoma） salivary gland tumors （P 〈 0. 05）. Conclusions Gadd45a mRNA expression level is decreased in salivary gland tumors, which may play an important role in the tumorigenesis of sali- vary gland tumors by inducing the tumors continue to proliferation.

关 键 词：GADD45A 涎腺肿瘤 RT-PCR 

分 类 号：R739.8[医药卫生—肿瘤]