束缚应激大鼠下丘脑BDNF和GAP-43变化及其与行为学变化的关系  

Expressions of hypothalamus BDNF and GAP-43 and their relation with behavior changes in response to restraint stress in rats

作　　者：戴若以[1] 张云东[2] 顾小红[1] 陈卓[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所老年科,重庆400042 [2]第三军医大学大坪医院神经外科,重庆400042

出　　处：《中华创伤杂志》2015年第2期169-172,共4页Chinese Journal of Trauma

基　　金：重庆市自然科学基金资助项目（cstc2013jjB10002）

摘　　要：目的 研究束缚应激对大鼠下丘脑脑源性神经生长因子（BDNF）和生长相关蛋白（GAP-43）表达变化及其与行为学变化的关系. 方法 将40只雄性SD大鼠按随机排列表法分为四组：对照组、束缚应激7d组、束缚应激14 d组、束缚应激21 d组,每组10只.通过对大鼠束缚制动建立应激模型,旷场实验观测行为学变化;ELISA法检测血浆促肾上腺皮质激素释放激素（CRH）浓度;Western blot检测模型大鼠下丘脑BDNF、GAP-43蛋白表达情况. 结果 束缚应激7d组在穿格次数（50.0±7.0）、站立次数（11.4±2.1）、修饰次数（11.2±2.7）上均高于其他各组（P＜0.05）.束缚应激14 d组和束缚应激21 d组在穿格次数（35.5±7.5,29.4±6.8）、站立次数（7.8±4.9,5.6±3.9）、修饰次数（6.7±2.9,4.4±2.6）均少于对照组（42.6±5.4,8.9±4.3,7.9±3.0）和束缚应激7d组（P＜0.05）.束缚应激7,14,21 d组血浆CRH浓度分别为（500.48±88.71） pg/ml、（750.73±123.68） pg/ml、（793.06±115.84） pg/ml,均明显高于对照组的（336.72±45.34） pg/ml（P ＜0.05）;束缚应激14 d组与束缚应激21 d组血浆CRH浓度高于束缚应激7d组应激组（P＜0.05）.对照组BDNF、GAP-43表达（0.672±0.185,0.694±0.253）最低,束缚应激后,随着时程的增加,其表达也逐步增加,在束缚应激21 d后达到最高值（1.357±0.524,1.486±0.679） （P ＜0.05）. 结论 束缚应激可致大鼠下丘脑BDNF、GAP-43蛋白表达上调,发生可塑性变化,可能与应激后行为学改变相关.Objective To investigate the expressions of brain-derived nerve growth factor （BDNF） and growth-associated protein 43 （GAP-43） in the hypothalamus of rats inflicted with restraint stress and their relationship with behavioral changes.Methods Forty male SD rats were divided into control group,restraint stress 7-day group,restraint stress 14-day group,restraint stress 21-day group according to the random number table,with 10 rats per group.Behavior changes were observed by open-field test,serum levels of corticotrophin releasing hormone （CRH） by enzyme-linked immunosorbent assay,and expressions of BDNF and GAP-43 in the hypothalamus by western blotting.Results Restraint stress 7-day group exhibited increases in spanning lattice times （50.0 ± 7.0）,standing times （11.4 ± 2.1）and modification times （11.2 ± 2.7） compared with all other groups （P 〈 0.05）.Restraint stress 14-day group and restraint stress 21-day group showed significant decreases in spanning lattice times （35.5 ±7.5,29.4 ± 6.8）,standing times （7.8 ± 4.9,5.6 ± 3.9） and modification times （6.7 ± 2.9,4.4 ±2.6） compared with control group （42.6 ± 5.4,8.9 ± 4.3,and 7.9 ± 3.0） （P 〈 0.05）.Restraint 14-day and 21-day groups showed significant increases in serum CRH level [（750.73 ± 123.68） pg/ml and （793.06 ± 115.84）pg/ml] compared with that in restraint stress 7-day group [（500.48 ± 88.71）pg/ml,P 〈0.05],but all were lower than （336.72 ±45.34） pg/ml in control group （P 〈0.05）.Levels of BDNF and GAP-43 in the hypothalamus were the lowest in control group （0.672 ± 0.185 and 0.694 ±0.253）.However,restraint stress increased the expressions of BDNF and GAP-43 in the hypothalamus,with the highest level in restraint stress 21-day group （1.357 ± 0.524 and 1.486 ± 0.679） （P 〈 0.05）.Conclusion Restraim stress can up-regulate BDNF and GAP-43 proteins in the hypothalamus,and lead to plasticity changes that may relate to stress-related behavior.

关 键 词：应激障碍 创伤性 下丘脑 促肾上腺皮质素 GAP-43蛋白 

分 类 号：R-332[医药卫生]