全反式维甲酸及其衍生物对乳腺癌细胞株MDA-MB-231凋亡的影响  

The influence of ATRA and its derivative on the cell apoptosis of breast cancer cell line MDA-MB-231

作　　者：周佳丽[1] 颜蕴文[1] 江巧玲[1] 吴燕[1] 周青[1] 汪渊[1] 

机构地区：[1]安徽医科大学分子生物学实验室、生物化学与分子生物学教研室、安徽省/省部共建教育部重要遗传病基因资源利用重点实验室,合肥230032

出　　处：《安徽医科大学学报》2015年第2期154-158,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81272399);安徽省自然科学基金(编号:090413116)

摘　　要：目的研究全反式维甲酸(ATRA)及其衍生物4-氨基-2-三氟甲基苯基维甲酸酯(ATPR)对人乳腺癌细胞MDAMB-231体外凋亡的影响。方法不同浓度ATRA及其衍生物ATPR分别处理MDA-MB-231细胞48 h后,Hoechst染色及流式细胞术检测细胞凋亡的变化,RT-PCR检测凋亡蛋白Caspase-3 mRNA水平的变化,Western blot法检测相关凋亡蛋白表达水平的变化。结果与ATRA相比,相同浓度的ATPR能明显促进MDA-MB-231细胞的凋亡,随着浓度的增加,凋亡作用越加显著(P<0.05)。RT-PCR显示ATPR作用后Caspase-3 mRNA水平显著上调(P<0.05)。Western blot法显示ATPR能下调抗凋亡蛋白Bcl-2、NF-κB、survivin的表达(P<0.05),上调促凋亡蛋白Bax、Grim-19、Caspase-3的表达(P<0.05)。结论 ATPR比ATRA更明显地促进人乳腺癌细胞MDA-MB-231的凋亡。Objective To investigate the influence of all-trans retinoic acid （ ATRA） and its derivative 4-amino-2-trifluoromethyl-phenyl ester （ATPR） on the apoptosis of breast cancer cell line MDA-MB-231. Methods MDA-MB-231 was treated with different concentrations of ATRA and ATPR for 48 h. Hoechst staining and flow cytometry were used to observe cell apoptosis. The mRNA level of apoptosis-related protein Caspase-3 was analyzed by RT-PCR. Western blot was performed to detect the expression of apoptosis-related proteins. Results Compared with ATRA, the same concentrations of ATPR promoted the apoptosis of MDA-MB-231 more obviously（P〈0. 05）, and the effect was dose-dependent. RT-PCR showed ATPR significantly raised the mRNA level of Caspase-3 （ P 〈0. 05 ） . Western blot displayed that ATPR decreased the expression of anti-apoptotic protein such as Bcl-2 , NF-κB, survivin（P 〈0. 05） and increased the expression of pro-apoptotic protein Bax, Grim-19, Caspase-3（P 〈0. 05）. Conclusion Contrasted to ATRA, ATPR is able to promote the cell apoptosis of MDA-MB-231 more markedly.

关 键 词：乳腺癌 MDA-MB-231 ATPR 凋亡 

分 类 号：R739.5[医药卫生—肿瘤] R966[医药卫生—临床医学]