Pro-BDNF及其受体在实验性变态反应性脑脊髓炎小鼠中枢神经系统的表达变化  

The expression of pro-BDNF and its receptor in central nervous system of experimental allergic encephalomyelitis of mouse

作　　者：胡招兰 曹文宇[1] 王雪琴[1] 段娟[1] 王洪涛[1] 张娟[1] 李芳[1] 李昌琪[1] 

机构地区：[1]中南大学基础医学院人体解剖学与神经生物学系,长沙410013

出　　处：《神经解剖学杂志》2015年第1期1-7,共7页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(31171151)

摘　　要：目的:探讨实验性变态反应性脑脊髓炎(EAE)的小鼠模型中枢神经系统中脑源神经营养因子前体(pro-BDNF)及其受体(Sortilin)的表达变化。方法:小鼠随机分为正常对照组和实验组,实验组小鼠上、下背部左右两侧及左、右侧尾根部分2次予以MOG35-55抗原多肽进行免疫,建立EAE模型。EAE组又分为发病前期(9 d)、发病初期(17 d)、发病高峰期(25 d)和发病缓解期(32 d)四组。采用免疫组织化学和Western Blot方法检测pro-BDNF及Sortilin在中枢神经系统(CNS)的表达变化。结果:pro-BDNF在EAE模型小鼠的皮质、胼胝体、海马、脊髓的表达与对照组相比,第9 d、第17 d出现下降,在发病高峰期即第25 d表达增加,在发病缓解期即第32 d时表达恢复至正常水平。Sortilin在EAE模型小鼠的皮质、胼胝体、海马、脊髓的表达与对照组相比,第9 d出现下降,在发病高峰期即第25 d表达增加,其他时间点与对照组相比差异无显著性。结论:pro-BDNF与Sortilin在EAE小鼠模型中枢神经系统的表达与其病情密切相关,提示它们可能参与EAE病理的发生发展。Objective： To investigate the expression of pro-BDNF and its receptor sortilin in central nervous system （CNS） of experimental allergic encephalomyelitis（EAE） mice. Methods：Mice were randomly divided into control group and EAE group. To establish EAE model, mice were subcutaneously injected with MOG35-55 peptide into the bilateral side of upper back, lower back and tail root twice. EAE mice were divided into four groups： earlier stage （9 d）, initial stage（ 17 d）, the peak stage（25 d） and remission stage （32 d）. The expression of pro-BDNF and sortilin in the CNS were tested by immunohistochemistry and Western Blot. Results ： Compared with the control group, the expression of pro- BDNF in the cortex, corpus callosum, hippocampus and spinal cord were decreaed at day 9 and 17, upregulated at day 25, and then returned to the baseline level at day 32. The expression of sortilin in the cortex, corpus callosum, hippocampus and spinal cord were reduced at day 9, and then increased at day 25 in EAE mice compared with control group, no significant differences were observed between the two groups at other illness stages. Conclusions： The expression of pro-BDNF and sortilin in the CNS is closely related to the state of illness in the EAE mice, suggesting that they may be involved in pathological development of EAE.

关 键 词：脑源性神经营养因子前体 实验性变态反应性脑脊髓炎 多发性硬化 小鼠 

分 类 号：R744.51[医药卫生—神经病学与精神病学]