人参皂甙Rd对成年大鼠脑梗死后新生血管生长的影响  

The effect of ginsenoside Rd on the growth of cerebral microvascular in adult rats after focal cerebral ischemia reperfusion

作　　者：惠娟[1] 曾联婷 赵永[1] 史明[1] 韩军良[1] 

机构地区：[1]第四军医大学西京医院神经内科,西安710032

出　　处：《神经解剖学杂志》2015年第1期13-18,共6页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(81073094)

摘　　要：目的：研究人参皂甙Rd （Ginsenoside Rd,GSRd）对成年大鼠脑梗死后新生血管生长的影响并探讨其可能机制.方法：96只成年雄性Sprague-Dawley大鼠,体质量280～ 300 g,采用线栓法阻断右侧大脑中动脉制作局灶性短暂性大脑中动脉栓塞模型（focal transient middle cerebral artery occlusion,MCAO）.随机分为4组：sham、sham＋ GSRd、MCAO对照组、MCAO＋ GSRd治疗组,每组24只,术后7d和14 d使用RECA-1单克隆抗体免疫组化法标记血管内皮细胞,计算缺血梗死区周围微血管密度和分支点,Western Blot方法测定脑缺血皮层血管内皮生长因子（vascular endothelial growth factor,VEGF）和缺氧诱导因子-1α（hypoxia-inducible factor 1 alpha,HIF-1 α）的蛋白表达水平.结果：（1）免疫组化结果显示：MCAO损伤7、14 d时,GSRd治疗组梗死区周围微血管密度分别为755.3±65.5/mm2和790.8±53.9/mm2,较单纯手术组（589.7±28.9/mm2和636.7±22.6/mm2）比较,均显著增多（P＜0.001）;而GSRd治疗组周围微血管分支点在7、14 d时分别为341.7±40.4/mm2和363.5±39.7/mm2,亦比单纯手术组（197.2±26.6/mm2和276.0±42.9/mm2）显著增多（P＜0.001）;（2）Western Blot结果显示：GSRd治疗组的VEGF及HIF-1α表达量显著高于单纯手术组和假手术组（P＜0.05）.结论：人参皂甙Rd显著促进大鼠脑梗死区血管生长,其机制可能与增强脑梗死后VEGF、HIF-1α的表达相关.Objective： To study the effect of gindenoside Rd （GSRd） on the growth of cerebral microvascular in adult rats after focal cerebral ischemia reperfusion and to explore its possible mechanism. Methods： 96 adult male Sprague- Dawley rats, weighting 280 -300 g, subjected to MCAO （focal transient middle cerebral artery occlusion） , were randomly divided into 4 groups ： sham, sham ＋ GSRd, MCAO control group, MCAO ＋ GSRd treatment group, 24 rats in ev- ery group. RECA, a immunohistochemistry monoclonal antibody of endothelial cells, was used to calculate cortical micro- vascular density and branching points on 7 d and 14 d after surgery. Western Blot was used for analysis the changes in ex- pression of vascular endothelial growth factor （VEGF） and hypoxia inducible factor-1α（ hypoxia-inducible factor-1 alpha, HIF-1α） in cerebral ischemic cortex. Results： （1） Immunohistochemical results showed that microvessel density around the infarction area in GSRd treatment group on 7 d and 14 d after operation was 755.3 ± 65.5/mm2 and 790.8 ± 53.9/mm2, respectively. Compared with the MCAO control group （589.7 ± 28.9/mm2 and 636.7 ±22.6/mm2 ）, GSRd treatment group was obviously increased （P 〈 0. 001 ）. On the other hand, vascular branch points around the infarction area in GSRd treatment group on 7 d and 14 d after operation were 341.7 ±40.4/mm2 and 363.5 ± 39.7/mm2 respectively. Compared with the MCAO control group（ 197.2 ± 26.6/mm2 和 276.0±42.9/mm2 ）, GSRd treatment group was sig- nificantly increased （P 〈 0.001 ） ; （2） The results of Western Blot displayed that among all 4 groups the expression of VEGF and HIF-1α increased obviously in GSRd treatment group （P 〈 0.05 ）. Conclusion： GSRd has a significant effect on cerebral microvascular growth in MCAO rats, the mechanism might be related to the increased expression of VEGF and HIF-1α after focal cerebral isehemia reperfusion.

关 键 词：人参皂甙RD 脑梗死 脑微血管 血管内皮生长因子 缺氧诱导因子1-Α 大鼠 

分 类 号：R743.3[医药卫生—神经病学与精神病学]