氟伐他汀对大鼠脑缺血再灌注损伤后Fas和FasL表达的研究  

The effects of fluvastatin preconditioning on expression of Fas and FasL in rats with focal cerebral ischemia-reperfusion

作　　者：赵红[1] 张国峰 张华[1] 付学锋[3] 万东君[3] 

机构地区：[1]空军总医院药学部,北京100142 [2]北京市通州区新华医院,北京101100 [3]兰州军区兰州总医院神经内科,兰州730050

出　　处：《神经解剖学杂志》2015年第1期49-53,共5页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金青年基金(81200247);全军医药卫生科研基金资助(CLZ11JB02);兰州军区医药卫生科研项目(CLZ11JB02)

摘　　要：目的:探讨氟伐他汀预处理对脑缺血再灌注损伤大鼠脑组织中Fas、FasL表达的影响。方法:实验大鼠随机分为假手术组(Sham组)、脑缺血再灌注组(I/R组)和氟伐他汀预处理组(Flu组)。线栓法制作大脑中动脉脑缺血再灌注模型,Flu组在模型制备前用氟伐他汀连续灌胃14 d。缺血2 h再灌注24 h后断头取脑,免疫组织化学法和半定量PCR法检测缺血区脑组织中Fas、FasL的表达。结果:通过免疫组织化学法和半定量PCR法检测大鼠缺血区脑组织中Fas和FasL阳性细胞,Sham组仅见少量表达,I/R组表达显著增多(P<0.05),Flu组表达显著减少(P<0.05)。大鼠缺血侧皮质区Fas mRNA和FasL mRNA表达为,Sham组有微弱的表达,I/R组表达显著增高(P<0.05),Flu组表达则显著降低(P<0.05)。结论:氟伐他汀对大鼠脑缺血再灌注损伤发挥保护作用的机制可能与下调Fas、FasL的表达有关。Objective： To explore the effects of fluvastatin preconditioning on the expression of Fas and FasL in rats with focal cerebral ischemia-reperfusion. Methods： Rats were randomly assigned into three groups： Sham operation group （Sham group） , focal cerebral ischemia-reperfusion group （I/R group） and fluvastatin reconditioning group （Flu group）. The middle cerebral artery occlusion reperfusion model was made by the suture method （ ischemia for 2 hours, and reperfusion for 24 hours）. The level of Fas and FasL expression were measured by immunohistochemistry and PCR. Results： By immunohistochemistry and semi quantitative PCR, the expressions of Fas and FasL positive cells in cerebral ischemic area were detected. The results showed that the expressions were a small amount in Sham group, expressions of I/R group increased significantly （ P 〈 0.05 ）, decreased significantly in group Flu group （ P 〈 0.05 ）. The expressions of Fas mR- NA and FasL mRNA for the rat cortex ischemia by immunohistochemistry and semi quantitative PCR, showed weak expressions in Sham group, increased significantly in I/R group （ P 〈 0.05 ） and decreased significantly in Flu group （P 〈 0. 05 ）. Conclusion： Fluvastatin might relieve the brain damage partly by decreasing Fas and FasL synthesis in the brain.

关 键 词：氟伐他汀 脑缺血再灌注 免疫组化 PCR FAS FASL 大鼠 

分 类 号：R741[医药卫生—神经病学与精神病学]