胃癌中VEGF、NRP1和干细胞标志物CD44的表达及相关性  

Expression of VEGF,NRP1 and cancer stem cell marker CD44 in gastric carcinoma and their correlations

作　　者：杨家佳[1] 张琳[1] 郑茂金[1] 王庆苓[1] 徐玉婷[1] 柳红[1] 

机构地区：[1]徐州医学院病理学教研室,徐州221000

出　　处：《临床与实验病理学杂志》2015年第2期124-127,131,共5页Chinese Journal of Clinical and Experimental Pathology

基　　金：江苏省属高校自然科学研究面上项目(12kJD320006)

摘　　要：目的探讨VEGF、NRP1和干细胞标志物CD44在胃癌组织中的表达及三者间的相关性。方法应用高通量组织芯片技术和免疫组化SP法检测72例胃癌和52例正常胃组织中VEGF、NRP1和CD44蛋白的表达,并分析三者之间的相关性以及与胃癌临床病理特征的关系。结果 (1)胃癌组织中VEGF、NRP1和CD44蛋白阳性率分别为76.4%、66.7%、83.3%,三者在胃癌组织中的表达显著高于正常胃组织,差异均具有统计学意义(P均<0.01)。(2)VEGF、NRP1蛋白表达与胃癌浸润深度、淋巴结转移、临床分期有关(P均<0.05);CD44表达与Lauren分型、分化程度、淋巴结转移、临床分期有关(P均<0.05)。(3)胃癌组织中VEGF、NRP1和CD44蛋白的表达均呈正相关(rs=0.578,rs=0.278,rs=0.316,P均<0.05)。结论 VEGF、NRP1和干细胞标志物CD44在胃癌中均呈高表达,且与胃癌的恶性生物学行为密切相关。VEGF与NRP1之间可能存在协同作用,二者均与干细胞标志物CD44的表达密切相关,其具体机制有待进一步研究。Purpose To investigate the expression of VEGF, NRP1 and the cancer stem cell marker CD44 in gastric carcinoma and their correlations. Methods High throughput technology of tissue microarray and immunohistochemical SP method were employed to investigate the expression of VEGF, NRP1 and CD44 proteins in 72 gastric carcinoma specimens and 52 normal gastric tissues. Immu-nohistochemistry records were reviewed, their correlations and the clinicopathological characteristics were evaluated. Results （ 1 ） The positive rates of VEGF, NRP1 and CD44 proteins were 76. 4%, 66. 7%, and 83. 3% in gastric carcinoma, respectively, with sig-nificant differences from normal gastric tissues （P〈0. 01）. （2） The positive expression of VEGF and NRP1 were closely correlated with infiltration depth, lymph node metastasis and TNM stage （P〈0. 05）. The positive expression of CD44 was closely correlated with Lauren type, differentiation degree, lymph node metastasis and TNM stage （P〈0. 05）. （3） In addition, the expression of CD44 was significantly correlated with VEGF and NRP1 in gastric carcinoma （rs =0. 578, rs =0. 278, rs =0. 316, P〈0. 05）. Conclusions VEGF, NRP1 and the cancer stem cell marker CD44 are highly expressed in gastric cancer, which are closely related to the biological behaviors of gastric cancer. Synergistic effects are observed between VEGF and NRP1, and they are closely related to the expression of the cancer stem cell marker CD44, which remains to be further studied.

关 键 词：胃肿瘤 VEGF NRP1 CD44 组织芯片 

分 类 号：R735.2[医药卫生—肿瘤]