MACC1在肝细胞癌中的表达与临床意义  

Expression of MACC1 in hepatocellular carcinoma and the relevant clinical significance

作　　者：吴华[1] 孟翔凌[1] 鲁贻明 吴维[2] 

机构地区：[1]安徽医科大学第一附属医院普外科,合肥230022 [2]蚌埠医学院第一附属医院肝胆外科,蚌埠233004

出　　处：《第二军医大学学报》2015年第2期167-171,共5页Academic Journal of Second Military Medical University

摘　　要：目的探讨肝细胞癌组织结肠癌转移相关基因1(metastasis-associated in colon cancer 1,MACC1)的表达及其临床意义。方法采用免疫组化测定108例病理确诊肝细胞癌患者的肝癌组织和癌旁无瘤肝组织中的MACC1蛋白表达情况,实时荧光定量PCR检测30例肝癌组织、癌旁组织以及20例正常肝组织中MACC1 mRNA的表达,分析肝癌组织中MACC1的表达水平与临床病理因素及预后的关系。结果免疫组化结果显示,肝癌组织中MACC1蛋白表达的阳性率为49.1%(53/108),高于癌旁肝组织34.2%(37/108,P=0.011);实时荧光定量PCR结果显示,MACC1mRNA在肝癌组织中的表达高于癌旁肝组织及正常肝组织(P<0.001)。单因素方差分析表明肝癌组织中MACC1的表达与肿瘤大小、有无包膜及病理分型密切相关(P<0.05)。生存分析表明MACC1阳性患者的1年、3年和5年总体生存率分别为0.650、0.492和0.280,而MACC1阴性患者1年、3年和5年总体生存率分别0.799、0.684和0.566,差异有统计学意义(P=0.006)。结论肝癌组织中MACC1的表达与肝癌的恶性演变及患者的临床预后密切相关,MACC1有望成为判断肝癌患者临床预后的重要分子,且有可能成为肝癌分子靶向治疗的新靶点。Objective To investigate the intratumoral expression of the metastasis-associated in colon cancer 1（MACC1）and to discuss the clinical relevance associated with hepatocellular carcinoma（HCC）.Methods Immunohistochemistry staining was performed to assess the expressions of MACC1 protein in 108 HCC liver tissues and matched adjacent non-tumor liver tissues.Quantilative real-time PCR（qPCR）was employed to examine MACC1 mRNA expression in 30 HCC tissues and the corresponding adjacent non-tumor tissue,and 20 normal liver tissues.The association of MACC1 expression in HCC tissues with the clinicopathological and prognosis was analyzed.Results Immunohistochemistry results showed that the positive rate of MACC1 in HCC tissues was significantly higher than that in the para-carcinoma tissue（49.1% vs 34.2%,P=0.011）.qPCR revealed that MACC1 mRNA expression in HCC tissues was significantly higher than that in the noncancerous liver tissues and normal liver tissues（P〈0.001）.One-factor analysis of variance indicated that MACC1 expression in human HCC tissue was significantly related to tumor size,presence of capsule and pathological types（P〈0.05）.Survival analysis disclosed that the1-year,3-year and 5-year survival rates were 0.650,0.492,and 0.280 for MACC1positive patients and 0.799,0.684,and0.566 for MACC1negative patients,respectively,with significant differences found between the two groups（P=0.006）.Conclusion Expression of MACC1 in HCC tissues is associated with malignancy evolution and clinical outcome of HCC patients;MACC1may serve as an important molecule for predicting prognosis patients with hepatic cancer and a new target for gene therapy of liver cancer.

关 键 词：结肠癌转移相关基因1 肝肿瘤 肝细胞癌 预后 

分 类 号：R735.7[医药卫生—肿瘤]