洛铂对卵巢癌裸鼠移植瘤的抑制作用和对凋亡相关基因调控的研究  

Inhibitory effects of lobaplatin on human ovarian cancer and its regulation of apoptosis-related genes in nude mice

作　　者：魏东敏[1] 于红艳[1] 王辉[2] 刘文利[1] 史素芳[1] 

机构地区：[1]河北工程大学附属医院妇产科,河北省邯郸056002 [2]解放军总医院妇产科

出　　处：《中国医师杂志》2015年第1期81-83,共3页Journal of Chinese Physician

摘　　要：目的探讨洛铂（LBP）、顺铂（DDP）对卵巢癌裸鼠移植瘤的生长抑制作用及对相关凋亡基因的调控。方法选用人卵巢癌细胞株SKOV3接种于裸小鼠皮下成瘤。将成瘤裸鼠按随机数字表法分为对照组、洛铂组和顺铂组，每组7只，洛铂组给予洛铂5mg／kg，顺铂组给予顺铂5mg／kg，于荷瘤成功后第1天、第8天和第15天经腹腔给药，每周3次记录瘤体体积和裸鼠体质量，并计算抑瘤率，用流式细胞学技术检测移植瘤组织的凋亡相关基因蛋白bax、bcl-2的表达。结果洛铂组的抑瘤率47．2％，顺铂组的抑瘤率42．8％，两组比较差异无统计学意义（P〉0．05）。洛铂组和顺铂组瘤组织中bax蛋白表达均高于对照组（P〈0．05），bcl-2蛋白表达均低于对照组（P〈0．05），但洛铂与顺铂组比较差异无统计学意义（P〉0．05）。结论洛铂具有抑制卵巢癌细胞生长的作用，其机制是通过调控凋亡基因bax和bcl-2的表达来实现的。Objective To explore the inhibitory effects of lobaplatin and cisplatin and their regulation of apoptosis-related genes in ovarian cancer cells in nude mice. Methods SKOV3 cells were implanted into nude mice. In monotherapy treatment study, the nude mice bearing human SKOV3 cells were randomly divided into control, lobaplatin, and cisplatin groups, with 7 mice in each group. The mice in each group were received corresponding treatment. The volume of tumor and the weight of nude mice were meas- ured three times per week, respectively. Tumor inhibitory rate was calculated. The protein expressions of bax and bcl-2 were detected by flow cytometry. Results The growth inhibitory rate was 47.2% in lobaplatin group and 42. 8% in cisplatin group, without signifi- cant difference between two groups （ P 〉 0. 05 ）. The expression of bcl-2 was decreased but the bax was increased in lobaplatin and ciaplatin groups compared to the control group. Conclusions Lobaplatin can significantly inhibit the growth of ovarian cancer cells, induce apoptosis by down-regulation of bcl-2 and up-regulation of bax.

关 键 词：有机铂化合物/药理学 疾病模型 动物 卵巢肿瘤/药物疗法 /小鼠 裸 肿瘤移植 基因 BCL-2 基因表达 细胞凋亡 

分 类 号：R737.31[医药卫生—肿瘤]