肿瘤标志物CD44v6、CD24与卵巢浆液性癌的关系  

Relationship Between Tumor Markers,CD44v6 and CD24 and Ovarian Serous Carcinoma

作　　者：伍宝珍 李惠[1] 

机构地区：[1]湖北省黄石市第四人民医院,湖北黄石435006

出　　处：《武汉大学学报（医学版）》2015年第2期235-238,共4页Medical Journal of Wuhan University

摘　　要：目的:观察和探讨肿瘤干细胞标志物CD44v6和CD24在卵巢浆液性癌中的表达特点,并分析其与临床病理参数和预后的关系。方法:选2009年6月-2013年6月就诊的卵巢浆液性癌进行手术切除的76例标本作为研究观察对象,采用两级病理分级系统对组织学分级进行判定,应用免疫组织化学EnVision二步法对癌组织中的CD44v6和CD24的表达进行检测,针对检查结果和对患者随访情况,对其与卵巢浆液性癌发展关系进行分析。结果:CD44v6阳性表达率为44.74%,主要表达于细胞膜;而CD24阳性表达率为63.16%,主要表达于细胞膜,可有腔缘表达;部分表达于细胞质,可有腔缘表达。两者均有表达共25例,比率为32.89%。CD44v6阳性与阴性之间生存率有显著差异(P<0.05);而CD24阳性与阴性之间生存率无显著差异(P>0.05)。CD44v6和CD24不同病理分级中表达有显著性差异(P<0.05),而在年龄、临床分级方面无显著相关性(P>0.05)。76例患者全部得到随访资料,其中随访复发45例,时间为手术后2-72个月之间,平均无瘤生存期为(33.5±12.7)月;共死亡21例,时间为术后6-57个月之间,平均生存时间为(43.6±17.6)月。CD44v6阳性与阴性病例相比较无瘤生存时间、死亡率和生存时间差异均显著(P<0.05);年龄为预后的独立影响因子(P<0.05)。结论:卵巢浆液性癌主要的不良因素为CD44v6和高龄,病理分级系统也与疾病预后有密切的关联。Objective：To observe the expression features of tumor stem cell markers CD24 and CD44v6in serous ovarian cancer and its relationship with clinicopathological parameters and prognosis.Methods：76patients with serous ovarian cancer who accepted surgical resection from June 2009 to June 2013 in our hospital were selected as research and observation objects.Histological grades were identified through two-grade histological grade determination,and expression of CD44v6 and CD24in the cancer tissues were tested with immunohistochemical EnVision method.The relationship between their expression and the development of serous ovarian cancer was studied.Results：Positive expression rate of CD44v6 was 44.74%,mainly expressed in the cell membrane,while positive expression rate of CD24 was 63.16%,mainly expressed in the cell membrane,with possible cavity edge expression,partially expressed in the cytoplasm,with possible cavity edge expression.There were 25 cases with both expressions,the ratio was 32.89%.There was a significant difference between CD44v6 and CD24expression in various pathological grade,with P0.05,and in age and clinical stage,there was no significant correlation with P0.05.All 76 patients were followed up,including 45 cases of recurrence,who occurred between 2and 72 months after surgery,the average tumor-free survival time was（33.5±12.7）months;There were 21 deaths,which occurred between 6and 57 months after surgery,and the average survival time was（43.6 ± 17.6）months.Tumor-free survival time,mortality,and survival time were different between positive and negative CD44v6,with P0.05;Age was an independent prognostic factor,with P0.05.Conclusion：Main adverse factors for serous ovarian cancer were CD44v6 and old age,and there are close relationship between pathological grading system and prognosis.

关 键 词：CD44V6 CD24 卵巢浆液性癌 表达 病理参数 预后 

分 类 号：R737.4[医药卫生—肿瘤]