ALDH2在白藜芦醇改善大鼠慢性酒精性肝损伤中的机制  

Resveratrol Protects against Chronic Alcoholic Liver Injury by Regulating Hepatic ALDH2 Expression in Rats

作　　者：黄丙清[1] 邱香[1] 王林枝[1] 朱腾世 郭琰芳 郝丽萍[1] 

机构地区：[1]华中科技大学同济医学院公共卫生学院营养与食品卫生学系,武汉430030

出　　处：《华中科技大学学报（医学版）》2015年第1期37-41,共5页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：国家自然科学基金资助项目(No.81172657)

摘　　要：目的探讨酒精代谢酶乙醇脱氢酶(ADH)和乙醛脱氢酶2(ALDH2)在白藜芦醇(resveratrol,Res)改善大鼠慢性酒精性肝损伤中的机制。方法采用含酒精的Lieber-DeCarli液体饲料喂养大鼠19周,建立慢性酒精性肝损伤模型。42只SD大鼠随机分成正常对照组,低、中、高剂量酒精组,Res对照组,高剂量酒精+Res组。采用苏木精-伊红(HE)和油红O染色,观察肝组织形态学改变;采用试剂盒测定肝组织中ADH、ALDH2的活性;Western blot检测肝组织中ADH与ALDH2的蛋白表达。结果各剂量酒精组大鼠肝组织脂肪变性和炎症浸润明显,体重和能量摄入量与正常对照组相比下降,差异具有统计学意义(P<0.05或P<0.01);且肝组织ADH活性与蛋白表达略有升高,而肝组织ALDH2活性与蛋白表达显著下降。Res干预后大鼠肝组织脂肪变性和炎症浸润均较高剂量酒精组显著减轻,体重与能量摄入量增加,显著提升了肝组织中ALDH2的活性与蛋白表达(P<0.05)。结论 Res可能通过对大鼠肝脏ALDH2的调节来改善慢性酒精摄入引起的肝脏损伤。Objective To evaluate the protective effects of resveratrol （Res）on chronic alcoholic liver injury in rats and fur‐ther explore the underlying mechanism involving hepatic ethanol metabolizing enzymes （ADH and ALDH2）.Methods Alcoholic liver injury models of rat were established by feeding Lieber‐DeCarli liquid ethanol diet for 19 weeks. Forty‐two SD rats were randomly divided into normal control group ,low‐,middle‐and high‐dose ethanol groups ,Res control group and high‐dose etha‐nol ＋Res group. The morphological change of hepatic tissues was observed by hematoxylin‐eosin（HE）and oil red O staining ;the activity of hepatic ADH and ALDH2 was determined by using appropriate kits ;and the protein expressions of hepatic ADH and ALDH2 were detected by Western blot.Results There were serious hepatic steatosis and inflammation in ethanol groups.Body weight and food intake were significantly decreased in the ethanol groups compared with the normal control group. There was moderate increase in the ADH activity and expression and significant decrease in ALDH 2 activity and expres‐sion in ethanol groups.Res could ameliorate ethanol‐induced hepatic steatosis and inflammation as well as increase body weight and energy intake. Hepatic ALDH2 activity and protein expression were significantly increased by Res treatment （P〈0.05） . Conclusion Res could improve chronic ethanol‐induced liver injury by regulating hepatic ALDH 2 expression.

关 键 词：酒精性肝损伤 白藜芦醇 乙醇脱氢酶 乙醛脱氢酶2 

分 类 号：R575[医药卫生—消化系统]