TYLCV山东分离物株系分化及侵染性克隆的构建  

Strain differentiation and infectious clone construction of tomato yellow leaf curl virus in Shandong Province

作　　者：薛东齐 刘冠[1] 姜景彬[1] 许向阳[1] 李景富[1] 

机构地区：[1]东北农业大学园艺学院,哈尔滨150030

出　　处：《东北农业大学学报》2015年第1期26-33,共8页Journal of Northeast Agricultural University

基　　金："十二五"国家科技计划项目(2012BAD02B02-7)

摘　　要：对2012~2014年山东省96份感番茄黄化曲叶病毒（TYLCV）样品进行特异性检测,发现有94份样品感染TYLCV,有2份样品感染番茄卷叶病毒（To LCV）。对该94份样品进行全序列克隆和序列测定,共分离出13份TYLCV株系,经核苷酸序列相似性分析和系统进化树构建,明确侵染我国山东地区的TYLCV为TYLCV-IL株系,且3年间爆发于山东省内的TYLCV株系的DNA组分并未发生大变异。遂构建山东寿光TYLCV株系侵染性克隆,经致病性验证,具有较好的侵染率,为番茄抗Ty育种提供稳定的毒源筛选压力。There were 96 samples of a sense of tomato yellow leaf curl virus（TYLCV） that were collected in Shandong Province between 2012- 2014. After specific detection, 94 samples infected TYLCV and two samples infected To LCV. The 94 samples that infected TYLCV were cloned and sequenced the whole sequence. The results showed that 13 TYLCV strains were separated. By nucleotide sequence similarity analysis and phylogenetic tree construction, We identified that the TYLCV isolates infected Shandong area belonged TYLCV-IL strains, and TYLCV DNA component of Shandong Province didn＇t occur large variation between 2012- 2014. Then we constructed Shandong Shouguang TYLCV strain infectious clone, By pathogenic verification, It had a better infection rate. Therefore, It provide a stable viral source of selection pressure for anti-Ty tomato breeding.

关 键 词：番茄黄化曲叶病毒 特异性检测 系统进化树 侵染性克隆 

分 类 号：S436.412.11[农业科学—农业昆虫与害虫防治]