箬竹叶提取物对烟草病原真菌的抑制作用  

Inhibitory Activity of Indocalamus Tessellatus Leaves Against Pathogenic Fungi of Tobacco

作　　者：李俊超[1,2] 胡坚[3] 杨春雷 胡国元[3] 余君 杨锦鹏 

机构地区：[1]湖北省烟草科学研究院,湖北武汉430030 [2]华中农业大学植物科技学院,湖北武汉430070 [3]武汉工程大学化工与制药学院,湖北武汉430074

出　　处：《化学与生物工程》2015年第2期43-47,共5页Chemistry & Bioengineering

基　　金：国家科技重点项目(中烟办[2013]212号)

摘　　要：采用菌丝生长速率法和孢子萌发法,对选用无水乙醇、冰乙酸和蒸馏水混合溶剂浸提所得的箬竹叶粗提物以及依次采用石油醚、乙酸乙酯和正丁醇进行液-液萃取所得的萃取物进行了抑菌活性测试。结果表明:箬竹叶粗提物对烟草链格孢菌和烟草疫霉菌菌丝生长均具有抑制活性,其对两种真菌抑制的EC50值分别为3.98mg·mL-1和0.88mg·mL-1、EC90值分别为7.33mg·mL-1和1.73mg·mL-1;对烟草链格孢菌孢子萌发抑制的EC50值和EC90值分别为4.68mg·mL-1和9.09mg·mL-1。2mg·mL-1的石油醚、乙酸乙酯、正丁醇萃取物和萃余水相对烟草链格孢菌菌丝生长的抑制率分别为31.27%、36.18%、6.06%和-7.05%;对烟草疫霉菌菌丝生长的抑制率分别为56.82%、81.53%、12.32%和-18.10%。乙酸乙酯萃取物对烟草链格孢菌和烟草疫霉菌菌丝生长的抑制活性明显高于箬竹叶粗提物,其对两种真菌抑制的EC50值分别为2.99mg·mL-1和0.80mg·mL-1、EC90值分别为5.41mg·mL-1和2.83mg·mL-1。乙酸乙酯萃取物对烟草链格孢菌孢子萌发抑制的EC50值和EC90值分别为1.03mg·mL-1和3.54mg·mL-1。After being dipped by ethanol,acetic acid and distilled water and then extracted with petroleum ether,ethyl acetate and n-butyl alcohol in sequence,the antifungal ingredients of Indocalamus tessellatus leaves were isolated.The antifungal activity was investigated by means of mycelium growth rate and spore germination.Results indicated that primary components of Indocalamus tessellatus leaves exhibited inhibitory activity to both fungus,the value of EC50 and EC90of primary components to mycelium growth rate of A.alternate were3.98mg·mL-1 and 7.33mg·mL-1;and to mycelium growth rate of P.parasitica were 0.88mg·mL-1 and1.73mg·mL-1;and to spore germination of A.alternate were 4.68mg·mL-1 and 9.09mg·mL-1.The inhibitory rate of extraction phase of petroleum ether,ethyl acetate,n-butyl alcohol and raffinate water phase with the concentration of 2mg·mL-1 to mycelium growth rate of A.alternate were 31.27%,36.18%,6.06% and-7.05%;and to mycelium growth rate of P.parasitica were 56.82%,81.53%,12.32% and-18.10%.The inhibitory activity of ethyl acetate extraction phase against pathogenic fungi of tobacco was obviously higher than primary components of Indocalamus tessellatus leaves.The value of EC50 and EC90of ethyl acetate extraction phase to mycelium growth rate of A.alternate were 2.99mg·mL-1 and 5.41mg·mL-1;and to mycelium growth rate of P.parasitica were 0.80mg·mL-1 and 2.83mg·mL-1;and to spore germination of A.alternate were 1.03mg·mL-1 and 3.54mg·mL-1.

关 键 词：箬竹 烟草链格孢菌 烟草疫霉菌 抑菌活性 

分 类 号：Q949.2[生物学—植物学]