土壤中产广谱抗菌肽菌株的筛选及鉴定  

Identification and Screening of Broad-spectrum Antibiotics-producing Strains from Soil

作　　者：唐峰[1] 李哲 王铁良[1] 唐雨顺[1] 刘永华[1] 寇叙[1] 刘秀萍[1] 

机构地区：[1]辽宁医学院畜牧兽医学院,辽宁锦州121001 [2]天津市津南区畜牧水产发展服务中心,天津300350

出　　处：《现代畜牧兽医》2015年第2期11-14,共4页Modern Journal of Animal Husbandry and Veterinary Medicine

基　　金：辽宁医学院横向课题(LYHX2012076);辽宁省教育厅科学研究一般项目(L2012306);辽宁省科技厅自然科学基金(联合基金)项目(2013022041)

摘　　要：用双层平板法从土壤中分离到4株具有广谱抑菌活性的菌株,利用琼脂扩散法测定其对鼠伤寒沙门菌、大肠杆菌、福氏志贺菌、金黄色葡萄球菌、黑曲霉菌的抑菌能力,筛选其中抑菌能力最强的菌株命名为JZ4。菌液上清分别经过盐析、透析或与蛋白酶作用后测定其抑菌能力,发现该抑菌物质中含有肽类物质。经16S r DNA序列比对,初步鉴定其为侧孢短芽孢杆菌。In this study, 4 strains with broad- spectrum antibacterial activity were isolated from soil with double-plate method, and the inhibition activity against Salmonella typhimurium, E.coli, Shigella, Staphylococcus aureus,and Aspergillus niger were determined by agar diffusion method. 1 strain named JZ4 with greater inhibition zone was identified. Salting-out, dialysis and protease experiment were carried out and then preliminary determined that the antibiotics were antibacterial peptide. According to the sequence of 16 S r DNA, strain JZ4 were identified as Brevibacillus laterosporus.

关 键 词：土壤 抗菌肽 抑菌 侧孢短芽孢杆菌 

分 类 号：S852.6[农业科学—基础兽医学]