磷酸化STAT3检测对肺腺癌所致恶性胸腔积液的诊断价值  

The significance of pSTAT3 test in the diagnosis of malignant pleural effusion induced by adenocarcinoma of lung

作　　者：田伊卿[1] 朱立强[2] 方先勇[2] 

机构地区：[1]江苏大学检验医学系,江苏镇江212013 [2]徐州医学院附属医院细胞室,江苏徐州221002

出　　处：《国际检验医学杂志》2015年第4期449-450,453,共3页International Journal of Laboratory Medicine

摘　　要：目的探讨磷酸化信号转导与转录激活因子3(pSTAT3)蛋白表达对肺腺癌所致恶性胸腔积液的诊断价值。方法应用液基细胞学技术,对临床送检的47例胸腔积液进行检测,同时每例均利用薄层细胞涂片行免疫细胞化学染色,检测pSTAT3蛋白的表达。结果 40例胸腔积液标本经临床及医学影像学资料和(或)组织学检查证实为肺腺癌所致恶性胸腔积液,7例为良性胸腔积液。pSTAT3在肺腺癌所致恶性胸腔积液中的阳性表达率为80.0%(32/40),高于良性组的0.0%(0/7),差异有统计学意义(P<0.05)。pSTAT3免疫细胞化学染色联合液基细胞学检查可使恶性胸腔积液的检出率达80.0%(32/40),高于单独使用液基细胞学检查的检出率[32.5%(13/40)],二者比较差异有统计学意义(P<0.05)。结论肺腺癌所致恶性胸腔积液中pSTAT3蛋白的表达明显增高,pSTAT3可作为肺腺癌所致恶性胸腔积液诊断的一个新的生物标记物,pSTAT3可能在胸腔积液的发生、发展中发挥重要作用。Objective To investigate the diagnostic value and clinical significance of phosphorylated signal transducer and activator of transcription 3（pSTAT3）in malignant pleural effusion（MPE）caused by lung adenocarcinoma.Methods 47 pleural effusion samples were collected and detected by using liquid-based cytologic test（LCT）.Furthermore,the expression of pSTAT3 was detected by using the method of immunocytochemistry in all of the specimens.Results Among the 47 cases of pleural effusion,40 cases of MPE were caused by lung adenocarcinoma and 7cases were benign pleural effusion,which were confirmed by clinical manifestation,biopsy and imaging data.The positive expression rate of pSTAT3 was 80.0%（32/40）in MPE caused by lung adenocarcinoma,significantly higher than benign group（P〈0.05）.13 cases of MPE caused by lung adenocarcinoma and 7cases of benign pleural effusion were diagnosed with liquid-based cytologic test alone.The other 27 cases were undetermined and needed to be differentiate between adenocarcinoma and atypical mesothelial cells.However,32 cases of lung adenocarcinoma and 7cases could be clearly diagnosed if liquid-based cytologic test was used combined with immunocytochemistry detection of pSTAT3 expression.Only 8cases were undetermined.Diagnosis of grey area was significantly narrowed.There was significant statistical significance between the two methods（P〈0.05）.Conclusion High expression of pSTAT3 is related to the MPE caused by lung adenocarcinoma.Detection of pSTAT3 expression can be used as a new method in the diagnosis of MPE caused by lung adenocarcinoma.pSTAT3 may play an important role in the development of the MPE.

关 键 词：肺腺癌 胸腔积液 免疫细胞化学 磷酸化信号转导与转录激活因子3 

分 类 号：R734.2[医药卫生—肿瘤] R730.43[医药卫生—临床医学]