负透镜诱导豚鼠视网膜中γ-氨基丁酸及其受体的表达  

Expressions of retinal γ-aminobutyric acid and its receptors in retina of guinea pig eyes after lens induction

作　　者：沙芳[1] 赵雯[1] 徐超立[1] 王玲[1] 张小燕[2] 吴建峰[1] 毕爱玲[3] 郭大东[3] 毕宏生[3] 

机构地区：[1]山东中医药大学,济南250002 [2]山东中医药大学第二附属医院眼科,济南250001 [3]山东中医药大学附属眼科医院山东中医药大学眼科研究所,济南250002

出　　处：《中华实验眼科杂志》2015年第2期123-128,共6页Chinese Journal Of Experimental Ophthalmology

基　　金：国家自然科学基金项目（81173440、81303081）;山东省科技发展计划项目（2011GGH21835、2011GGB14097）;山东省医药卫生科技发展计划项目（2011HD014）;济南市高校自主创新计划项目（201102061）

摘　　要：背景 研究表明,抑制性氨基酸神经递质γ-氨基丁酸（GABA）在近视的发生和发展过程中发挥关键作用,GABA及GABA受体拮抗剂对近视发生的抑制作用已被证实,但相关作用机制的研究鲜见报道.目的 探讨负透镜诱导性近视（LIM）豚鼠视网膜中GABA及其受体亚型表达的变化,探讨GABA及GABA受体拮抗剂抑制近视发生的机制.方法 采用随机数字表法将40只3周龄健康三色短毛豚鼠随机分为正常对照组和模型组.正常对照组取右眼作为正常对照眼,模型组右眼佩戴-10 D透镜4周以诱导近视模型作为实验眼,左眼不戴镜作为对照眼.分别于造模前及造模后4周用A型超声法和带状光检影法测量各组豚鼠的眼轴长度和屈光度;于造模后4周断颈法处死豚鼠并分离视网膜,采用高效液相色谱分析（HPLC）法检测豚鼠视网膜中GABA含量的变化,采用SYBR Green Ⅰ实时荧光定量PCR检测豚鼠视网膜中GABA受体mRNA的相对表达量. 结果 造模后4周,模型组实验眼、正常对照眼和模型组对照眼屈光度分别为（-3.39±0.70）、（1.03±0.68）和（0.77±0.64）D,模型组实验眼近视屈光度较正常对照眼和模型组对照眼均明显增大,差异均有统计学意义（t=-16.382、-17.113,均P＜0.05）;模型组实验眼、正常对照眼和模型组对照眼眼轴长度分别为（8.62±0.08）、（8.31±0.05）和（8.32±0.05） mm,模型组实验眼眼轴较正常对照眼和模型组对照眼明显延长,差异均有统计学意义（t=10.981、15.838,均P＜0.05）;豚鼠正常对照眼、模型组实验眼、模型组对照眼视网膜中GABA含量分别为（14.83±1.20）、（18.63±1.58）和（15.74±0.96） μmol/g,模型组实验眼视网膜中GABA含量明显高于正常对照眼和模型组对照眼,差异均有统计学意义（t=-6.045、6.876,均P＜0.05）;模型组实验眼视网膜中GABAA受体、受体GABAC受体mRNA的相对表达量明显高于正常对照眼,差异均有统计学�Background Researches showed that gamma aminobutyric acid （GABA）,an inhibitory amino acid neurotransmitter,plays a critical role in myopia refractive development.The inhibitory roles of GABA and its receptors antagonists have been confirmed.However,the study on whether GABA participates in the mechanisms of lens-induced myopia （LIM） in guinea pigs is rare.Objective The aim of this study was to explore the alteration of expression of GABA and its three receptor subtypes in the retinas of guinea pigs with LIM.Methods Threeweek-old healthy guinea pigs were randomized into the normal control group and the model group.The right eyes of the guinea pigs in the normal control group served as the normal control eyes,and the right eyes of the guinea pigs in the model group monocularly fitted with-10 D lens as the experimental eyes,and the fellow eyes of the model group were used as the self-control eyes.The refraction and axial length of eyes were measured by A-sonography and streak retinoscopy before and 4 weeks after modeling.The guinea pigs were sacrificed at the fourth week after modeling,and the retinas were isolated.GABA content in the retinas was assayed by high performance liquid chromatography （HPLC） and the relative expression levels of subtypes of GABA receptors were detected by real-time PCR.All the results were compared among the groups.All experimental protocols and animal handling procedures were approved by the Animal Care and Use Committee of Shandong University of Traditional Chinese Medicine,and were in accordance with the statement of the Association for Research in Vision and Ophthalmology for the use of animals.Results At the fourth week after modeling,the mean diopter of the model eyes was （-3.39±0.70）D,which was significantly higher than （1.03±0.68） D of the normal control eyes and （0.77 ±0.64） D of the self-control eyes in the model group （t =-16.382,-17.113,both at P〈0.05）.The mean axial length of the experimental eyes was （8.62±0.08）mm,which was increased in

关 键 词：近视/透镜诱导性 Γ-氨基丁酸 Γ-氨基丁酸受体 视网膜 

分 类 号：R778.11[医药卫生—眼科]