雌激素代谢酶CYP1A1及COMT基因多态性与子宫肌瘤相关性研究  

Association of Gene Polymorphisms of CYP1A1 and COMT with Uterine Leiomyomas

作　　者：王晓莉[1] 曾静[1] 王珏[2] 王璟[1] 胡婷[1] 王敏[1] 

机构地区：[1]四川省成都市第二人民医院,四川成都610017 [2]中国医学科学院输血研究所,四川成都610000

出　　处：《实用妇产科杂志》2015年第2期120-123,共4页Journal of Practical Obstetrics and Gynecology

基　　金：四川省卫生厅科研基金资助项目(编号:070375)

摘　　要：目的:探讨雌激素代谢酶CYP1A1和COMT基因多态性与子宫肌瘤发生的关系。方法:应用聚合酶链反应-限制性片段长度多态性分析(PCR-RFLP)技术,对100例子宫肌瘤患者和100例对照组人群CYP1A1基因Msp I位点多态和COMT基因外显子4密码子158(G-A)多态性进行分析。结果:1两组均存在CYP1A1基因Msp I位点多态,但两组基因型TT、TC、CC频率和等位基因T、C频率的比较差异均无统计学意义(P>0.05);2两组均存在COMT基因G-A多态,但两组基因型GG、GA、AA频率和等位基因G、A频率的比较差异均无统计学意义(P>0.05)。结论:CYP1A1基因Msp I位点多态性和COMT基因外显子4密码子158(G-A)多态性与子宫肌瘤发病风险无相关性。Objective： To explore the association of CYP1A1 and COMT polymorphisms with uterine leiomyoma.Methods： The T-C polymorphism of CYP1A1 gene and the G-A polymorphism in exon 4 codon 158 of COMT gene were detected by polymerase chain reaction-restricition fragment length polymorphism（ PCR-RFLP） in a case-control study,including 100 cases of uterine leiomyomas（ study group） and 100 cases as controls（ control group）. Results： 1 The T-C polymorphism of the CYP1A1 gene was shown clearly both in study and control groups,but the genotype distributions（ TT、TC and CC） and allele frequencies（ T and C） of CYP1A1 polymorphism were of no significant difference between the two groups（ P〉0. 05）; 2The G-A polymorphism of the COMT gene was shown clearly both in study and control groups,but the genotype distributions（ GG、GA and AA） and allele frequencies（ G and A） of COMT polymorphism were of no significant difference between the two groups（ P 〉0. 05）. Conclusions： The T-C polymorphism of CYP1A1 gene and the G-A polymorphism of COMT gene are not associated with the occurrence risk of uterine leiomyomas.

关 键 词：子宫肌瘤 细胞色素P450 1A1 COMT基因 遗传多态性 

分 类 号：R737.33[医药卫生—肿瘤]