顺铂联合HSV-tk自杀基因对肝癌细胞HepG2的生长抑制作用  

Inhibition of cisplatin combined with HSV-tk suicide gene in proliferation of HepG2 hepatoma cells

作　　者：张继宏 莱智勇 张甜甜[2] 徐钧[3] 

机构地区：[1]山西省孝义市人民医院普外科,032300 [2]山西省临汾市人民医院普外科 [3]山西省人民医院普外科

出　　处：《中国药物与临床》2015年第2期154-156,共3页Chinese Remedies & Clinics

基　　金：国家自然科学基金(81172136)

摘　　要：目的研究HSV-tk自杀基因联合顺铂对人Hep G2细胞株的杀伤作用。方法使用脂质体携带p AFP-TK-IRES2-EGFP质粒转染Hep G2细胞;Hep G2细胞在转染HSV-TK基因后,继续添加顺铂,用CCK-8试剂检测HSV-tk基因联合顺铂对Hep G2细胞杀伤效果。结果重组质粒p AFP-tk-IRES2-EGFP成功转染Hep G2肝癌细胞;HSV-tk自杀基因联合顺铂对Hep G2细胞的杀伤作用大于单独使用顺铂或HSV-tk自杀基因(P<0.05)。结论实验成功评价了顺铂联合HSV-tk自杀基因对Hep G2肝癌细胞株的杀伤效果,为肝癌的临床治疗方法提供了实验依据。Objective To investigate the killing effect of HSV-tk suicide gene combined with cisplatin in human Hep G2 cell line. Methods The p AFP-TK-IRES2-EGFP plasmid was carried by liposome to transfect Hep G2 cells. After HSV-tk gene was transfected by Hep G2 cells, cisplatin was added and then CCK-8 reagent was used to determine the killing effects of HSV-tk gene combined with cisplatin in Hep G2 cells. Results Recombinant plasmid p AFP-TK-IRES2-EGFP plasmid successfully transfected Hep G2 hepatoma cells. The killing effect of HSV-tk suicide gene combined with cisplatin in Hep G2 cells was greater than using cisplatin or HSV-tk suicide gene alone（P0.05）.Conclusion Our findings successfully assessed the killing effect of cisplatin combined with HSV-tk suicide gene in Hep G2 hepatoma cell line, providing evidence for clinical treatment for hepatoma.

关 键 词：肝肿瘤 顺铂 胸苷激酶 

分 类 号：R735.7[医药卫生—肿瘤]