甲状旁腺激素（1-34）联合珊瑚转化型羟基磷灰石促进种植体周围骨缺损再生修复的实验研究  

Effect of parathyroid hormone（1-34） and coralline hydroxyapatite on bone regeneration of peri-implant bone defects

作　　者：薛妍[1,2] 张惠利[1] 车彦海[1] 蔡青[1] 周延民[1] 孟维艳[1] 

机构地区：[1]吉林大学口腔医学院种植中心,长春130021 [2]海南省人民医院口腔科,海口570102

出　　处：《中华口腔医学杂志》2015年第2期99-102,共4页Chinese Journal of Stomatology

摘　　要：目的 探究甲状旁腺激素（1-34） [parathyroid hormone（1-34）,PTH（1-34）]联合珊瑚转化型羟基磷灰石（coralline hydroxyapatite,CHA）对种植体周围骨缺损处骨再生修复的影响.方法 在8只杂种犬的两侧胫骨各制备两个种植窝,于种植窝的一侧骨壁创造骨缺损.将种植体植入种植窝,CHA植入骨缺损处.术后将8只犬分为实验组和对照组,每组各4只.实验组每周连续3d背部皮下注射40 μg/kg PTH（1-34）溶液,对照组以同样方法注射等量生理盐水.术后4和8周两组各处死2只动物获取标本,进行生物力学测试、X线片和组织学观察.结果 术后4、8周实验组骨缺损处的密度影均高于对照组,种植体与骨接触的界面间未见低密度透射影像;实验组种植体最大拔出力值（199.8、411.5 N）均显著高于同期的对照组（100.1、184.5 N）,P＜0.05;拔出力值实验组术后4周与对照组术后8周接近.术后4周实验组骨缺损区新生骨小梁较对照组稍粗,连续性较好;种植体表面与新生骨直接接触.术后8周实验组新生骨较对照组致密;种植体表面与新生骨接触较对照组广泛.结论 PTH（1-34）联合CHA可促进种植体周围骨缺损再生修复,使种植体与骨愈合周期缩短.Objective To evaluate the effect of parathyroid hormone（1-34） [PTH（1-34）] and coralline hydroxyapatite（CHA） on bone regeneration of peri-implant bone defects.Methods Two implant sites were prepared on both sides of tibia in 8 mongrel dogs.The bone defect was created along one bone wall of each implant site.Implants were implanted into the implant sites,then CHA was grafted into the bone defects.After surgery,the animals were randomly divided into two groups.PTH（1-34） （40 μg/kg） was used for subcutaneous injection to the experimental group for three consecutive days,meanwhile the same amount of saline was given to the control group.Half of the animals of each group were sacrificed after 4 weeks and 8 weeks respectively.Specimens were subjected to implant pull-out strength tests,X-ray picture and histological observation.Results The bone density of bone defects in the experimental group were higher than that in the control group.No low-density images was observed between the implants and bone at 4 weeks and 8 weeks.The maximum pull-out force value of the experimental group（199.8 N,411.5 N） was higher at 4 weeks and 8 weeks than that of the control group（100.1 N,184.5 N） （P〈0.05）.The pull-out force value of the experimental group at 4 weeks and the pull-out force value of the control group at 8 weeks were similar.The new bone trabecular around CHA of experimental group was thicker at 4 weeks.Implant surface contacted to the new bone directly without fiber.CHA granules of the experimental group at 8 weeks were fewer than that of the control group.New bone tissue of the experimental group was denser.The contact area between implant surface and new bone was wider in experimental group than in the control group.Conclusions PTH（1-34） and CHA can promote bone regeneration of peri-implant bone defects,shorten the implants and bone healing cycle and improve the implants osseointegration.

关 键 词：牙种植 羟基磷灰石类 甲状旁腺激素相关蛋白质 

分 类 号：R783[医药卫生—口腔医学]