一株Bacillus sublitisJH-1发酵产木聚糖酶培养基的响应面优化  

Optimization of Bacillus sublitis JH-1 for Xylanase Production by Response Surface Analysis

作　　者：王春明[1] 钟超[1] 王凤学[1] 黄凡[1] 贾红华[1] 韦萍[1] 赵印[2] 

机构地区：[1]南京工业大学生物与制药工程学院,南京211816 [2]河南天冠企业集团有限公司车用生物燃料技术国家重点实验室,南阳473000

出　　处：《生物技术通报》2015年第2期179-186,共8页Biotechnology Bulletin

基　　金：国家"863"计划(2012AA021405);车用生物燃料技术国家重点实验室开放课题(2013024);广西科学研究与技术开发计划(桂科重1348004-4)

摘　　要：利用刚果红透明圈法从秸秆堆肥中筛选出一株产木聚糖酶菌株Bacillus sublitis JH-1,以单因素试验为基础,采用响应面试验设计对Bacillus sublitis JH-1液态发酵产木聚糖酶的培养基进行了优化,得到产木聚糖酶发酵最适条件为:培养温度33℃,p H值6.0,麦芽糖浓度为2.6%,酵母粉浓度为1.2%,KH2PO4浓度为1.2%,发酵产酶量比优化前提高了1.8倍。Xylanase producing strain Bacillus sublitis JH-1 was screened from straw compost by Congo red transparent circle method. According to the single-factor experiments, response surface methodology was applied to optimize the liquid state fermentation conditions of Bacillus sublitis JH-1 for xylanase production, with the optimal conditions being listed as follows：temperature as 33℃, pH value as 6.0, maltose as 2.6%（m/V）, YE as 1.2%（m/V）, KH2PO4 as 1.2%（m/V）. Increased 1.8 times in average as compared with preliminary culture and consistent with the maxmum predicted value.

关 键 词：BACILLUS sublitis JH-1 木聚糖酶 响应面法 

分 类 号：Q936[生物学—微生物学]