Silica sol/Nano-Au/PVP修饰金电极电致化学发光测定槐定碱  

Determination of Sophoridine by Electrochemiluminescence Based on Nano-Au / Silica sol / PVP Modified Gold Electrode

作　　者：李彦青[1] 罗应[2] 杨兰兰[1] 程昊[1] 黄文艺[1] 李利军[1] 

机构地区：[1]广西科技大学生物与化学工程学院,广西柳州545006 [2]广西科技大学鹿山学院,广西柳州545616

出　　处：《分析测试学报》2015年第2期210-215,共6页Journal of Instrumental Analysis

基　　金：国家自然科学基金资助项目(20665001);广西自然科学基金资助项目(0640029);广西高校科学技术研究项目(2013YB176);广西科技大学科学基金项目(1261115;1419217)

摘　　要：基于槐定碱对联吡啶钌（Ru（bpy）3^2＋）在电致化学发光中的增敏机理,通过传感器修饰技术,将Silica sol/Nano-Au/PVP修饰到金电极表面,建立了简单、快捷测定槐定碱的新方法。实验表明,最佳实验条件下,在1.6×10^-7-1.6×10^-4mol/L范围内,槐定碱的浓度与其对应的电化学发光强度值呈良好的线性关系,I=4.000×10^7c＋262.2,r^2=0.994 5,检出限（S/N=3）为3.5×10^-9mol/L。连续平行测定浓度为1.6×10^-5mol/L的槐定碱溶液6次,其相对标准偏差（RSD）为2.9%。该方法具有较高的选择性和灵敏度,样品处理简单快速,用于槐定碱的测定,结果满意。In order to establish a simple and rapid method for the determination of sophocarpine,the gold electrode modified with silica sol / Nano-Au / PVP was prepared,which could be used to detect sophoridine by ECL based on the obvious electrochemiluminescence sensitization effect of sophoridine on Ru（ bpy）2 ＋3. Under the optimum conditions,the ECL intensities presented a good linearity with concentration of sophoridine in the range of 1. 6 × 10^- 7- 1. 6 × 10^- 4mol / L,with a linear equation of I = 4. 000 × 10^7 c ＋ 262. 2（ r^2= 0. 994 5） and a detection limit（ S / N = 3） of 3. 5 × 10^- 9mol / L. RSD（ n = 6） for determination of 1. 6 × 10^- 5mol / L sophoridine was 2. 9%. The proposed method showed a high selectivity,sensitivity and simplicity,and could be applied in the analysis of sophoridine with satisfactory results.

关 键 词：电化学发光 联吡啶钌 硅溶胶 纳米金 槐定碱 

分 类 号：O657.1[理学—分析化学] TQ460.72[理学—化学]