石榴皮总多酚脂质体制备工艺参数的统计学分析  

Optimization of Preparation Process of Pomegranate Peel Polyphenols Liposomes

作　　者：王占一[1] 戴博 王玉海[1] 张立华[1] 李卓瓦[1] 毕海丹[1] 

机构地区：[1]枣庄学院生命科学学院,山东枣庄277160 [2]空军总医院,北京100142

出　　处：《中国实验方剂学杂志》2015年第5期33-37,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家星火计划项目(2012GA740049);山东省自然科学基金项目(ZR2013BL018)

摘　　要：目的:探究应用超临界CO2流体沉析技术制备石榴皮总多酚脂质体的工艺条件,为该制剂的工业化生产提供参考。方法:以包封率为评价指标,通过Plackett-Burman试验筛选主要影响因素,利用最陡爬坡试验和Box-Behnken试验优选石榴皮总多酚脂质体的制备工艺。结果:原药与膜材的质量比、反应温度和反应压力是影响总多酚脂质体包封率的显著性因素,最佳制备工艺条件为原药-膜材的质量比1∶21.15,反应温度48.3℃,反应压力23.88 MPa。包封率实测值80.295%和预测值81.379%的偏差1.33%,有效粒度168.5 nm,Zeta电位-73.8 m V。结论:该试验模型设计达到要求,工艺具有可行性。石榴皮总多酚脂质体体外释放速度缓慢,缓释效果明显,稳定性良好。Objective： To optimzie preparation process of pomegranate peel polyphenols liposomes by using supercritical CO2 fluid precipitation technology. Method： With entrapment efficiency as index, main factors were screened by Plackett-Burman test, preparation process of pomegranate peel polyphenols liposomes was optimized by steepest ascent test and Box-Behnken test. Result： Ratio of raw materials and membrane material, reaction temperature and pressure were significant factors, optimal preparation conditions was as following： ratio of raw materials and membrane material of 1：21. 15, reaction temperature at 48.3 ℃ , reaction pressure of 23.88 MPa. The measured value and the predicted value of entrapment efficiency were 80. 295% and 81. 379%, effective size was 168.5 nm, Zeta potential was -73.8 inV. Conclusion： This test model meets requirements. In vitro release of pomegranate peel polyphenols liposomes is slow, its sustained release effect is obvious.

关 键 词：石榴皮 总多酚 脂质体 最陡爬坡试验 包封率 

分 类 号：R283.6[医药卫生—中药学] R195.1[医药卫生—中医学]