多种植物提取物对DMPD自由基清除作用的研究  

The study on the DMPD free radical scavengation by some plant extract

作　　者：刘国安[1] 常锦春[1] 乔想金 何彩丽[1] 邹柯姝[1] 丁兰[1] 

机构地区：[1]西北师范大学生命科学学院,兰州730070

出　　处：《华中师范大学学报（自然科学版）》2015年第1期102-107,共6页Journal of Central China Normal University：Natural Sciences

基　　金：国家自然科学基金项目(30960464);教育部留学回国人员科研启动基金项目

摘　　要：用DMPD法检测了植物来源的天然产物对DMPD˙+自由基的清除作用.结果表明,11种单体化合物和沙棘籽油在此反应体系表现出不同的活性.黄酮类化合物中槲皮素活性最强,100μmol/L时清除率为55.76%,芦丁和葛根素居中,分别是16.64%、2.25%,芹菜素最弱,此浓度未检测到清除作用;酚类化合物中阿魏酸的活性最强,100μmol/L时达96.78%;三萜化合物熊果酸较齐墩果酸作用强,1μmol/L时检测到清除活性达到最高,分别为23.05%和3.05%;混合物沙棘籽油对DMPD˙+自由基有一定的清除能力,其半清除浓度为12.84mg/mL.此结果为这些植物来源化合物发挥抗氧化作用的合理浓度范围及进一步应用提供了一定的依据.The DMPD assay was used for evaluating scavenging effect of several botanical products on free radical DMPD˙＋.The results indicated that 11 kinds of monomeric compounds and sea-buckthorn seed oil exhibited different antioxidant activities in this system.In flavonoids group,quercetin displayed the strongest scavenging capacity,apigenin showed the weakest,rutin and puerarin were medium,at 100μmol/L,the clearance rate was 55.76%,16.64%,2.25%and non-scavenging,respectively.As phenolic compounds,ferulic acid showed the strongest action,the clearance rate reached 96.78%at 100μmol/L.Triterpenoids ursolic acid exhibited more effective than oleanolic acid,at 1μmol/L they exhibited the highest scavenging rate,23.05% and 3.05%,respectively.Sea-buckthorn seed oil had the definite effect,the half clear concentration is12.84mg/mL.The results provided date to the further use and reasonable concentration in application of these compounds.

关 键 词：DMPD 自由基清除作用 植物抗氧化剂 

分 类 号：Q591[生物学—生物化学]