UC001kfo对肝癌细胞细胞骨架蛋白表达的调控效应  

Regulatory effect of long non-coding RNA UC001kfo on the expression of cytoskeletal protein in hepatocellular carcinoma cells

作　　者：潘延凤[1] 郑岩[1] 秦涛[2] 张耀丹[1] 伍孝贤[1] 李花花[1] 

机构地区：[1]郑州大学第一附属医院感染科,450052 [2]河南省人民医院肝胆胰腺外科

出　　处：《中华实验外科杂志》2015年第2期300-301,共2页Chinese Journal of Experimental Surgery

摘　　要：目的 观察新基因UC001 kfo对肝癌细胞细胞骨架蛋白表达的调控效应.方法 以肝癌细胞株HepG2为细胞模型,采用脂质体转染技术分别将UC001kfo-pCDNA和pCDNA转染至HepG2细胞内,分为UC001 kfo-pCDNA、pCDNA、HepG2组,每组设3个复孔,实时定量聚合酶链反应（Real-time PCR）检测UC001 kfo、α-平滑肌肌动蛋白（α-SMA）mRNA表达,免疫组织化学及积分吸光度（IA）分析α-SMA的表达.结果 Real-time PCR结果显示48 h后UC001kfo表达量在UC001 kfo-pCDNA组、pCDNA组、HepG2组分别为1 924.14±238.69、1.87 ±0.43、1.00±0.29,UC001 kfo-pCDNA组与阴性对照组和空白对照组比较差异有统计学意义（P＜0.01）;α-SMA表达量分别为6.81 ±1.39、0.82±0.14、1.00±0.54,UC001 kfo-pCDNA组与阴性对照组和空白对照组比较差异均有统计学意义（P＜0.01）.48 h UC001kfo-pCDNA、pCDNA、HepG2组IA值分别为972.53±59.10、623.11±57.66、697.98±51.29,UC001 kfo-pCDNA组与阴性对照组比较差异有统计学意义（P＜0.01）,与空白组比较差异无统计学意义（P＞0.05）.结论 UC001 kfo上调α-SMA的表达,促进肝癌细胞的侵袭转移.Objective To explore the regulatory effect of the new gene UC001kfo on the expression of cytoskeletal protein in hepatocellular carcinoma cells.Methods The hepatocellular carcinoma cell line HepG2 was used as the cell model,the liposomal transfection technique was applied to transfect the UC001kfo-pCDNA and pCDNA into the HepG2 cells respectively,and three groups were set up：UC001kfo-pCDNA group,pCDNA group and HepG2 group （three holes for each group）.Real-time quantitative polymerase chain reaction （Real-time PCR） was used to detect the expression of UC001kfo,and α-smooth muscle actin （ot-SMA） mRNA.Immunohistochemistry and the integral absorbance （IA） were used to analyze the expression of α-SMA.Results Real-time PCR showed that the expression of UC001kfo in UC001kfo-pCDNA group,pCDNA group and HepG2 group was 1924.14 ±238.69,1.87 ± 0.43 and 1.00 ± 0.29 respectively,and there was statistically significant difference between UC001 kfo-pCDNA group and pCDNA group or HepG2 group （both P 〈0.01）.α-SMA expression in UC001kfo-pCDNA group,pCDNA group and HepG2 group was 6.81 ± 1.39,0.82 ±0.14 and 1.00 ±0.54 respectively,and there was statistically significant difference between UC001kfo-pCDNA group and pCDNA group or HepG2 group （both P 〈0.01）.The IA values in UC001kfo-pCDNA group,pCDNA group and HepG2 group were 972.53 ±59.10,623.11 ±57.66 and 697.98 ±51.29 respectively,and there was statistically significant difference between UC001 kfo-pCDNA group and pCDNA group or HepG2 group （P 〈 0.01）,but no statistically significant difference was found between pCDNA group and HepG2 group （P 〉 0.05）.Conclusion UC001 kfo can increase the expression of α-SMA and promote the invasion and migration of hepatocellular carcinoma cells.

关 键 词：癌 肝细胞 细胞骨架蛋白 UC001kfo Α-平滑肌肌动蛋白 

分 类 号：R735.7[医药卫生—肿瘤]