钙蛋白酶小亚基-1与肾透明细胞癌转移相关性及其对细胞侵袭能力的影响  

The relationship between calpain small subunit 1 gene and clear cell renal cell carcinoma and its influence to invasiveness of 786-0 cells

作　　者：庄乾锋[1] 何小舟[1] 许贤林[1] 徐仁芳[1] 范敏[1] 崔笠[1] 

机构地区：[1]苏州大学附属第三医院泌尿外科,常州213003

出　　处：《中华实验外科杂志》2015年第2期414-416,共3页Chinese Journal of Experimental Surgery

摘　　要：目的 探讨钙蛋白酶小亚基-1 （Capn4）与肾透明细胞癌（ccRCC）转移的相关性及其表达对肾癌细胞侵袭能力的影响.方法 应用采用实时定量聚合酶链反应（Real-time PCR）及免疫组织化学方法检测75例ccRCC及癌旁组织中Capn4的表达.采用合成的小干扰RNA质粒转染786-0细胞,检测转染后Capn4的表达及786-0细胞的侵袭能力.结果 ccRCC组织中Capn4的表达明显高于癌旁组织（2.125±0.793比1.480±0.584,P＜0.05）,且单因素及多因素分析显示,与淋巴结转移呈正相关[比值比（OR）=0.598,95％置信区间（CI）0.389 ～0.918,P＜0.05].Capn4基因降表达后786-0细胞的侵袭数量明显下降[（166±52）比（448±47）个,P＜0.05].结论 Capn4基因与ccRCC的转移相关,其表达抑制能降低786-0细胞的侵袭能力.Objective To research the relationship between calpain small subunit 1 （Capn4） gene and clear cell renal cell carcinoma and the influence of Capn4 expression change to invasiveness of 786-0 cells.Methods Capn4 expression was measured in 75 pairs of clear cell renal cell carcinoma （ccRCC） and matched adjacent non-tumor tissues by real-time quantitative polymcrase chain reaction （Real-time PCR） and immunohistochemistry.Specific small interfering RNA （siRNA） for Capn4 gene was designed and synthesized,and then 786-0 cells were transfected with siRNA-Capn4 to inhibit the expression of Capn4 gene.After RNA interference,invasiveness of 786-0 cells was detected.Results Capn4 expression in the ccRCC tissues was significantly higher than in the adjacent non-tumor tissues （2.125 ± 0.793 vs.1.480 ± 0.584,P 〈 0.05） ; the expressions of Capn4 were closely related with lymphatic metastasis of clear cell renal cell carcinomain [odds ratio （OR） =0.598,95% confidence interval （CI） 0.389-0.918,P 〈 0.05].After RNA interference,the invasiveness of 786-0 cells reduced significantly （166 ± 52 vs.448 ± 47,P 〈 0.05）.Conclusion There is a relationship between Capn4 gene with metastasis of renal carcinoma,and the inhibition of Capn4 gene expression can inhibit the invasiveness of 786-0 cells.

关 键 词：肾透明细胞癌 转移 侵袭 

分 类 号：R737.11[医药卫生—肿瘤]