裸鼠原位高转移胰腺癌模型的建立  

Establishment and study of highly metastatic pancreatic cancer model in nude mice

作　　者：张毓青[1] 徐新生[1] 崔乃强[1] 

机构地区：[1]天津市南开医院肝胆胰外科,300100

出　　处：《中华实验外科杂志》2015年第2期422-423,共2页Chinese Journal of Experimental Surgery

基　　金：国家科技支撑计划资助项目（2006BAI04A15）

摘　　要：目的 研究一种成功率高、转移率高的人胰腺癌原位动物造模方法.方法 将MIAPaCa-2细胞接种到裸鼠的皮下,待其成瘤后,再将皮下瘤块无菌条件下取出,原位种植到30只裸鼠胰腺尾部,于4、6、8周分批处死裸鼠,进行大体及组织学观察和分析.结果 本研究裸鼠胰腺原位成瘤率达100％,苏木素-伊红（HE）染色观察发现4、6、8周裸鼠原位成瘤的胰腺癌细胞形态学上无明显差异,均为低分化胰腺癌细胞.镜下证实原位植瘤后4周裸鼠远处转移率为0％,原位移植瘤后8周远处转移率为100％,包括腹腔淋巴结转移及远处脏器转移,两者差异有统计学意义（P＜0.05）.肝转移仅为1例,占10％.结论 裸鼠4周成瘤属于胰腺癌的早期,肿瘤未发生转移;8周成瘤属于胰腺癌晚期,肿瘤发生了淋巴结及远处转移,且利用该种建模方法成功率高、转移率高、肝转移率低.Objective To study a method of orthotopic transplantation model of pancreatic cancer with high success rate and high transfer rate.Methods This research used the MIA PaCa-2 cell lines to establish a nude mouse orthotopic transplantation model of pancreatic cancer.The cancer cells were subcutaneously injected into 30 nude mice,after being tumor,removed subcutaneous tumor with sterile conditions,grew into nude mice pancreas tail in situ.Morphological and biological features,and metastasis of the transplanted tumor were studied after 4,6 and 8 weeks.Results In this study the rate of tumor was 100%,and there was no obvious difference from the cells morphology of pancreatic tumor between 4 weeks and 8 weeks with hematoxylin and eosin （HE） staining,there were poorly differentiated pancreatic cancer cells.The distant metastasis rate was 0% in postoperative 4 weeks by the microscopic observation,distant metastasis in nude mice was 100% in postoperative 8 weeks,including local lymph node metastasis and distant organ metastasis,there were extremely significant statistical differences （P 〈 0.05）.The hepatic metastasis rate was 10% only.Conclusion That showed tumor of 4 weeks belonged to pancreatic cancer early in the disease process,and tumor of 8 weeks belonged to the late stages of pancreatic cancer disease process,it is high success rate,high transfer rate and low hepatic metastasis rate of using this method to build the orthotopic transplantation model.

关 键 词：胰腺癌 裸鼠 原位模型 高转移 

分 类 号：R735.9[医药卫生—肿瘤]