Id1基因表达与垂体腺瘤的侵袭性研究  

Study of the expression of Id1 gene to invasion of the pituitary adenoma

作　　者：马勃[1] 李志强[2] 沈赞[3] 

机构地区：[1]安徽理工大学病原免疫学教研室,安徽省淮南232001 [2]上海交通大学附属第六人民医院南院上海市奉贤区中心医院神经外科 [3]上海交通大学附属第六人民医院肿瘤科

出　　处：《中国基层医药》2015年第1期1-4,161,共5页Chinese Journal of Primary Medicine and Pharmacy

基　　金：国家自然科学基金资助项目（81172105）

摘　　要：目的 探讨侵袭性垂体腺瘤的发病机制,提高临床治疗效果.方法 从所收集的114例标本中选取15例侵袭性垂体腺瘤组织和15例非侵袭性垂体腺瘤组织,采用定量RT-PCR、Western blot及免疫组化方法,检测Id1基因和Id1蛋白在侵袭性垂体腺瘤组织中的表达和其在非侵袭性垂体腺瘤组织中的表达;利用小片段干扰RNA（small interfering RNA）干扰Id1基因在NQ-04细胞中的表达.结果 Id1基因在侵袭性垂体腺瘤组织中的表达明显高于非侵袭性垂体腺瘤组织（n=15,t=2.725,P=0.013）;干扰Id1组细胞的迁移能力显著减弱.结论 Id1基因在侵袭性垂体腺瘤组织中表达显著上调,可能与其侵袭性相关.Id1有望成为评估垂体腺瘤预后的生物学标志物.Objective To explore the pathogenesis of invasive pituitary adenomas,and to improve the effect of clinical treatment.Methods 15 pairs of invasive pituitary adenoma tissues and noninvasive pituitary adenoma tissues were selected from 114 specimens collected and the Id1 gene expression and Id1 protein expression in invasive pituitary adenoma tissues and noninvasive pituitary adenoma tissues were tested by quantitative RT-PCR,Western blot and immunohistochemical methods;Then small interfering RNA （siRNA） was used to interfer Id1 gene expression in NQ-04 cells.Results The Id1 gene expression in invasive pituitary adenoma tissues was significantly higher than that in noninvasive pituitary adenoma tissues（n =15,t =2.725,P =0.013） ;The migration of pituitary adenoma cells interfered was significantly reduced.Conclusion The invasive pituitary adenoma tissues has high level of Id1 gene expression,which may be correlated with its invasion.And Id1 is expected to become a biological marker of diagnosis and determination in the prognosis of pituitary adenoma.

关 键 词：Id1基因 垂体腺瘤 侵袭性 

分 类 号：R736.4[医药卫生—肿瘤]