肝性脑病大鼠脑组织中基质金属蛋白酶-9的表达变化及意义  

Expression of matrix metalloproteinases-9 in the brain tissue of experimental hepatic encephalopathy rats

作　　者：胡立华[1] 陈鹏[1] 王晓丽[1] 张继晔[1] 张艳杰[1] 张沛怡[1] 

机构地区：[1]黑龙江省医院消化内科,黑龙江省哈尔滨150036

出　　处：《中国基层医药》2015年第2期175-177,共3页Chinese Journal of Primary Medicine and Pharmacy

基　　金：黑龙江省卫生厅科研项目（2013360）

摘　　要：目的 研究基质金属蛋白酶-9（MMP-9）的表达在肝性脑病发病中的作用和意义.方法 180只大鼠按完全随机化原则分为对照组、肝性脑病4h、8h、12 h组,每组45只,应用D-氨基半乳糖（400 mg/kg）和内毒素（100 μg/kg）联合腹腔内注射建立肝性脑病大鼠模型.分别于造模后4h、8h、12 h检测血氨浓度、伊文思蓝（EB）含量、脑组织含水量及Real-time Q-PCR法检测血脑屏障MMP-9 mRNA含量.结果 造模2h后肝性脑病组大鼠逐渐出现肝性脑病症状.造模后4h大鼠血氨浓度即开始升高[（74.27±1.77） μmol/L比（33.85±1.50）μmol/L,F=5 983.36,P＜0.01],且随时间延长血氨浓度升高更加明显[（136.32±3.88）μmol/L、（279.53±9.78） μmol/L,F=5 983.36,P＜0.01];造模后4h大鼠脑组织EB含量即开始升高[（4.84±0.43） μg/g比（3.96 ±0.48） μg/g,F=111.54,P＜0.01],且随时间延长EB含量升高更加明显[（5.94±0.35）μg/g、（6.71±0.50） μg/g,F=111.54,P＜0.01];造模后4h大鼠脑组织含水量即开始升高[（78.63±0.46）％比（76.07±0.39）％,F=737.25,P＜0.01],且随时间延长脑组织含水量升高更加明显[（80.50±0.32）％、（82.14±0.30）％,F=737.25,P＜0.01];造模后4h大鼠脑组织MMP-9 mRNA表达的相对含量即开始升高[（1.037 ±0.071）比（0.829±0.053）,F=132.10,P＜0.01],且随时间延长脑组织MMP-9mRNA表达的相对含量升高更加明显[（1.235±0.095）、（1.425 ±0.113）,F=132.10,P＜0.叭].结论 肝性脑病时出现血脑屏障通透性增强及脑水肿,可能与MMP-9 mRNA表达升高有关.Objective To investigate the significance of MMP-9 expression in the pathogenesis of hepatic encephalopathy.Methods 180 rats were randomly divided into the control group,hepatic encephalopathy 4,8 and 12h groups,45 rats in each group.In hepatic encephalopathy group,rats were intraperitoneally injected D-galactosamine （400mg/kg） with lipopolysaccharide （100μg/kg）,and then the symptom was observed.Ammonia,the evans blue（EB） content,the brain water content and the MMP-9 mRNA in the brain tissue were assessed by real-time Q-PCR after 4,8 and 12h.Results 2h later the rats in hepatic encephalopathy groups began to appear the symptom.4 hours later,ammonia,the EB content,the brain water content and MMP-9 mRNA content of the hepatic encephalopathy groups began to increase [（74.27 ± 1.77） μ mol/L vs （33.85 ± 1.50） μ mol/L,F =5 983.36 ;（4.84 ±0.43）μg/g vs （3.96 ±0.48）μg/g,F =111.54; （78.63 ± 0.46）% vs （76.07 ±0.39）%,F =737.25,and （1.037 ± 0.071） vs （0.829 ± 0.053）,F =132.10,P 〈 0.01],and with the extension of time increased obviously [（136.32 ± 3.88） μmol/L and （279.53 ± 9.78） μmol/L,F =5 983.36 ; （5.94 ± 0.35） μg/g and （6.71 ±0.50）μg/g,F=111.54;（80.50±0.32）% and （82.14±0.30）%,F=737.25;（1.235 ±0.095） and （1.425± 0.113）,F =132.10,P 〈 0.01].Conclusion Blood brain barrier permeability enhancement and brain edema in hepatic encephalopathy rats may be related with MMP-9 mRNA increasing.

关 键 词：肝性脑病 血脑屏障 基质金属蛋白酶-9 

分 类 号：R575.3[医药卫生—消化系统]