两种阿尔茨海默病转基因小鼠模型脑新皮质区Aβ分布的比较研究  

Comparison of the Distribution of Aβ Immunoreactive in the Neocortex of the Two Kinds of Transgenic Mice Model of Alzheimer's Disease

作　　者：周奕 张剑峰[2] 卢璨[2] 彭凤兰 艾卫敏[3] 雷德亮[4] 

机构地区：[1]长沙卫生职业学院基础学科部,湖南长沙410100 [2]中南大学湘雅医学院 [3]湘潭职业技术学院护理学院人体解剖学教研室,湖南湘潭411012 [4]中南大学基础医学院人体解剖学与神经生物学系,湖南长沙410013

出　　处：《现代生物医学进展》2014年第34期6625-6628,共4页Progress in Modern Biomedicine

基　　金：长沙市2013年度指导性科技计划(K13ZD046-33);湖南省卫生厅科研基金课题(B2014-151);湖南省教育厅科学研究项目(14C009);米塔尔学生创新项目(10MX19)

摘　　要：目的:比较Aβ在两种阿尔茨海默病转基因小鼠模型脑新皮质区分布的差异。方法:采用18月龄雄性APP/PSl双转基因(2×Tg-AD)小鼠与同龄同性别APP/PSl/tau三转基因(3×Tg-AD)小鼠,取新皮质区脑组织行6E10单克隆抗体免疫组化染色等方法显示Aβ阳性神经元及斑块,观察其分布与形态等的差异,图像分析系统定量比较其量的变化。结果:在新皮质区2×Tg-AD组Aβ阳性产物主要位于细胞外即细胞外Aβ(e Aβ),形成大量的老年斑,细胞内阳性产物少;而3×Tg-AD组Aβ阳性产物主要位于神经元细胞内即细胞内Aβ(i Aβ),但老年斑少见。结论:2×Tg-AD组与3×Tg-AD组Aβ阳性产物在新皮质区分布的差异可能反映了两种AD小鼠模型神经病理等改变的不同。Objective： To compare the distribution and morphological features of Aβ Immunoreactive products in the neocortex of the APP/PSl（2×Tg-AD） and APP/PSl/tau（3×Tg-AD） model of Alzheimer＇s disease. Methods： 18-month old mice of 2×Tg-AD and the age-matched mice of 3×Tg-AD were included in this experiment. In the neocortex, 6E10 immunohistochemical staining was applied to show the distribution and morphological features of Aβ immunoreactive products, and image analysis system was applied to quantitatively analyze the difference of the products. Results： The deposition of Aβ in the neocortex of 2×Tg-AD was mainly seen as extracellular Aβ（e Aβ）, and formed a lot of positive plaques. The positive products were distributed as intracellularly Aβ（i Aβ） for the3 ×Tg-AD, but the Aβ positive plaques were rarely seen. Conclusion： The differences of distribution of Aβ positive products in the neocortex between the 2 ×Tg-AD and 3 ×Tg-AD mice likely suggested the differences of neuropathological changes of the two AD models.

关 键 词：阿尔茨海默病 转基因 新皮质区 Β淀粉样蛋白 

分 类 号：R741.02[医药卫生—神经病学与精神病学]