西藏青稞抗旱相关基因表达模式分析研究  

The Drought-related Gene Expression Pattern Analysis in Tibetan Barley

作　　者：原红军[1,2] 

机构地区：[1]西藏自治区农牧科学院农业研究所,西藏拉萨850002 [2]西藏自治区青稞种质改良和牦牛繁育重点实验室

出　　处：《现代农业科技》2015年第3期26-28,共3页Modern Agricultural Science and Technology

基　　金：973计划前期研究专项(2012CB723006);国家科技支撑计划(2012BAD03B01);西藏财政专项(2014CZZ001)

摘　　要：从青稞苗期叶片干旱胁迫诱导基因表达的正向差减文库中筛选出丝氨酸/苏氨酸蛋白激酶基因(AAPK)、胁迫诱导蛋白基因(Tsi1)、脱水蛋白基因(Dehydrin)、Δ1-二氢吡咯-5-羧酸合成酶基因(P5Cs)等9个与抗旱相关的基因,采用实时荧光定量PCR对其表达模式进行分析。结果表明,在渍水条件下,Tsi1、ERA1、Os SKIPa、Nt-SYR1、SHMT和AAPK基因上调表达,而P5Cs、Dehydrin基因下调表达;随着干旱胁迫处理时,Tsi1、ERA1、Os SKIPa和AAPK基因也呈上调表达,干旱复水后8 h时,这些基因表达量均达到最高。Nt-SYR1干旱胁迫后,在土壤绝对含水量达到4.8%明显上调表达,复水后2 h时表达量最高;P5CSs基因,在严重干旱胁迫下呈下调表达,在干旱后复水后表达量逐步上调表达;Dehydrin基因在干旱胁迫处理时上调表达,随着复水时间增加,也呈上调表达;SHMT基因在干旱处理里及复水后均而呈下调表达;SAMDC基因在渍水及正常生长所需的土壤绝对含水量时表达无变化,干旱处理时呈下调表达,但土壤绝对含水量达到4.8%明显上调表达,之后又呈下调表达,整个处理时期表达量较低。A forward subtracted library was used to select out the serine/threonine protein kinase gene （AAPK）, stress-reduced protein gene （ Tsil ）, dehydrated protein gene （Dehydrin）,△1-PYRROLINE-5-earboxylie acid synthase gene （P5Cs）and other 9 genes associated with drought by suppression subtractive hybridization using seedling leaf of Tibet harley,and used real-time quantitative PCR analysis of its expression pattern.The results showed that under waterlogged conditions, Tsil, ERA 1, OsSKIPa,Nt-SYR1 ,SHMT and A A PK genes upregulated, and P5Cs ,Dehydrin genes downregnlated.With drought stress treatment, Tsil, ERA 1, OsSKIPa and AA PK genes was upregulated, drought complex after 8 h of water, expression of these genes reached highest levels.After Nt-SYR1 drought stress ,the expression of genes was significantly upregnlated when the absolute water content in the soil reached 4.8 percent,the expression reached highest after rehydration for 2h.P5Cs gene under severe drought stress were downregulated, the expression was upregnlated gradually after rehydration.Dehydrin genes upregulated during drought stress treatment,with rehydration time increased, also showed upregnlated.SHMT genes in drought years and rehydration was downregulated.The soil absolutely moisture of SA MDC gene did not change in normal growth and watedogging,the drought was downregnlated ,but the soil absolute moisture content （4.8%）was significantly upregulated, and later was downregnlated, expression was ]：ow levels throughout the treatment period. Key words barley; drought stress ; gene ; Real Time PCR ;Tibetan

关 键 词：青稞 干旱胁迫 基因 REAL Time PCR 西藏地区 

分 类 号：S512.3[农业科学—作物学]