凉血活血方对肝星状细胞LX-2增殖和凋亡的影响  

Effect of Liang Xue Huo Xue Fang on the Proliferation and Apoptosis of LX-2 Cells

作　　者：李录[1] 马力天[1,2] 杨明会[3] 周刚[1] 张毅[2] 刘卓锋[2] 周咏春[4] 吴德喜[1] 白杨[1] 郑瑾[2,3] 

机构地区：[1]第四军医大学学员旅,陕西西安710032 [2]第四军医大学唐都医院中医科暨中西医结合肿瘤科,陕西西安710032 [3]解放军总医院中医院,北京100037 [4]第四军医大学西京医院放疗科,陕西西安710032

出　　处：《现代生物医学进展》2014年第33期6401-6403,6459,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金面上项目(81072973);省级中医药临床研究课题(Lc70)

摘　　要：目的:探讨凉血活血方(LiangXue Huo Xue Fang)对肝星状细胞LX-2增殖和凋亡的影响。方法:体外培养肝星状细胞LX-2,分别将凉血活血方(浓度为1.125,2.228,3.31,4.37,5.41 mg·mL-1),作用于LX-2细胞后24 h,48 h用MTT比色法检测细胞的增殖情况,选取合适的药物浓度(4,8,12,16 mg·mL-1),用流式细胞术检测加药24 h后细胞的凋亡率。结果:1MTT法显示不同浓度凉血活血方作用LX-2细胞24 h,48 h后,除24 h,1.125 mg·mL-1和2.228 mg·mL-1外,其余组增殖抑制率均明显高于正常对照组(P<0.05)。2流式细胞术检测显示,凉血活血方浓度为4,8,12,16 mg·mL-1)时,可明显促进LX-2细胞凋亡。结论:一定剂量的凉血活血方能抑制LX-2细胞的增殖,可能与其促进细胞凋亡有关。Objective： To investigate the effect of Liang Xue Huo Xue Fang on the proliferation and apoptosis of LX-2 cells.Methods： LX-2 cells were treated with different concentrations of Xue Huo Xue Fang（1.125, 2.228, 3.31, 4.37, 5.41 mg·mL^-1）. Cell proliferation was measured by MTT after LX-2 cells were treated with Liang Xue Huo Xue Fang for 24 h and 48 h. Then, cell apoptosis was detected at 24 h by flow cytometry in a appropriate drug concentration（4, 8, 12, 16 mg·mL^-1）. Results： ①Except 1.125 mg·mL^-1and2.228 mg·mL^-1in 24 h, cell proliferation in other centration groups was dramatically inhibited by Liang Xue Huo Xue Fang（P〈0.05）,and the inhibition rate was higher than control group. ②Liang Xue Huo Xue Fang could promote apoptosis of LX-2 cells at 4,8,12,16mg·mL^-1. Conclusion： A certain concentration of Liang Xue Huo Xue Fang can inhibit the proliferation and apoptosis of LX-2 cells.

关 键 词：凉血活血方 LX-2细胞 增殖 凋亡 

分 类 号：R575.2[医药卫生—消化系统] R285.5[医药卫生—内科学]