miRNA在白血病干细胞中的表达谱  

Expression profile of microRNAs in human leukemic stem cells

作　　者：耿其荣[1] 文海军[2] 陈晓勤[1] 王华[1] 王亮[1] 王维达[1] 吕跃[1] 

机构地区：[1]中山大学肿瘤防治中心,华南肿瘤学国家重点实验室血液肿瘤科,广州510060 [2]中山大学肿瘤防治中心,华南肿瘤学国家重点实验室实验研究部,广州510060

出　　处：《广东医学》2014年第20期3150-3152,共3页Guangdong Medical Journal

基　　金：广东省科技计划项目(编号:2011B061300070)

摘　　要：目的观察miRNA在人骨髓白血病干细胞中的表达情况。方法以从人骨髓中分离培养的骨髓白血病干细胞为实验对象,用Mir Va Na试剂盒分离提取细胞内小RNA。用Agilent 2100分析仪分析其完整性和含量。用Agilent人miRNA V12芯片分析miRNA的表达。结果复苏后存活的单核细胞经流式分选出的白血病干细胞群比例为1.58%,细胞数为2.5×105个。分选出的白血病干细胞抽提得到3μg的总RNA。利用安捷伦miRNA芯片一共检测到81个人源miRNA的表达。其中,表达量最高的10个miRNA分别是miR-233、miR-21、miR-142、miR-720、miR-23a、let-7f、let-7a、miR-16、miR-15a和miR-19b。利用Target Scan软件,预测这10个表达量最丰富的miRNA共有2 147个靶标基因。初步进行功能富集分析,发现这些miRNA所调控的基因主要集中在转录调控、生物合成、跨膜运输、免疫系统发育、淋巴系和髓系细胞分化等方面。结论筛选出了在人骨髓白血病干细胞中表达的miRNA,为其参与调控白血病干细胞的增殖分化提供了依据,同时为miRNA在白血病干细胞中潜在应用奠定基础。Objective To identify the expression of miRNAs in leukemic stem cells （LSCs）. Methods LSCs were obtained from patients with acute myeloid leukemia （AML）. Small molecular RNAs were extracted using mirVana miRNA isolation kit. The miRNA expression was analyzed with Aligent miRNA profiling assay. Results A total of 2.5 × 10^5 LSCs were isolated from AML bone marrow, and 3 μg of total RNA were obtained. According to Aligent microarray screening, 81 human miRNA were obtained. The top 10 were mir - 233, mir - 21, mir - 147, mir - 720, mir - 23a, ].et -7f, let -7a, mir - 16, mir - 15a and mir - 19b. Conclusion These findings indicate that a large diverse population of miRNAs are likely important regulators for LSCs in maintaining their state of differentiation, and have potential value in stem cells research.

关 键 词：MIRNA 急性髓系白血病 白血病干细胞 

分 类 号：R733.71[医药卫生—肿瘤]