Alk-SMase基因敲除小鼠的饲养繁殖及鉴定  

Breeding and identification of Alk-SMase knockout mice

作　　者：陈橼[1] 吴金东[1] 钱俊[2] 朱顺星[3] 邵冰峰[1] 张素青[1] 何向锋[1] 杨俐萍[1] 张一心[1] 

机构地区：[1]南通大学附属肿瘤医院,江苏南通226001 [2]南通大学研究生部 [3]实验动物中心,江苏南通226001

出　　处：《南京医科大学学报（自然科学版）》2015年第2期183-187,共5页Journal of Nanjing Medical University(Natural Sciences)

摘　　要：目的 :为了繁育和鉴定碱性鞘磷脂酶(Alkaline sphingomyelinase,alk-SMase)基因敲除小鼠,将引进的基因敲除小鼠进行饲养繁殖,杂合子、纯合子用于继续保种。方法:对其幼鼠剪尾提取基因组DNA,采用PCR方法进行基因型鉴定;取alkSMase表达组织做HE染色进行形态学比较。结果 :对引进小鼠已成功饲养和繁殖,并得到纯合alk-SMase基因缺失型小鼠。结论:正确的饲养、繁殖及基因鉴定方法对于基因敲除小鼠的获得和保种具有重要的意义。Objective：To breed and identify of alkaline sphingomyelinase （Alkaline sphingomyelinase, alk-SMase）knockout mice. nethods：DNA was extracted from the mice tissue. PCR method was used for genotype identification. HE staining was used for morphology identification. Results：The gene kwockout mice has been successfully feed and breeded,and the generated alk-SMase knockout（KO） mice were gotten. Conclusion：The correct way to feed,breed and gene identification for gene knockout mice has important significance for obtainmont and transgenic mice preservation.

关 键 词：alk-SMase 基因敲除小鼠 PCR 

分 类 号：R735.35[医药卫生—肿瘤]