透钙磷石涂层浸提液掺锶对MC3T3-E1细胞VEGF、βFGF和Runx2的影响  

Influences of extract of brushite coating mixed with strontium on cells VEGF, βFGC and Runx2 in MC3T3-E1

作　　者：文臣娟[1] 梁永强[2] 赵坚[1] 韩东颖[3] 

机构地区：[1]河北省唐山市开滦总医院,063000 [2]河北联合大学博创口腔医院 [3]唐山市中心血站

出　　处：《现代口腔医学杂志》2015年第1期30-34,共5页Journal of Modern Stomatology

摘　　要：目的通过体外细胞学实验对透钙磷石与锶二者协同作用于小鼠成骨细胞进行相关研究,为优化锶浓度提供理论依据。方法利用电化学沉积法制备含透钙磷石涂层的钛片,再利用浸提方法制备不同含锶量的浸提液。将MC3T3-E1细胞于浸提液中进行培养,利用RT-PCR技术检测各组细胞因子:血管生长因子(VEGF)、成纤维细胞生长因子β(βFGF)和侏儒蛋白相关转录因子2(Runx2)的表达情况。结果透钙磷石对成骨标志物的表达有促进作用,而掺锶透钙磷石则更能促进这一作用。其与锶的浓度有关,当锶浓度为0.5%时其对成骨细胞的作用最明显。结论透钙磷石具有良好的生物活性,具有良好的诱导成骨细胞分裂、分化的作用。而向透钙磷石中掺入不同浓度的锶,可以在一定程度上促进细胞VEGF、βFGF和Runx2的表达,从而明显增强其对成骨细胞分化的诱导作用。Objective In vitro ceil-based experiments were used to investigate the synergy between brushite and strontrium on osteoblasts in mice to provide theoretical references for the optimization of the strontrium concentration. Methods Titanium sheets containing a brushite coating was prepared through a kind of electrochemical deposition method and then extracts containing different levels of strontrium were prepared through extraction. MC3T3-E1 cells were incubated in the extracts. The expressions of the various cytokines such as the vascular endothelial growth factor （VEGF）, the fibroblast growth factor β （βFGF） and the runt-related transcription factor 2 （Runx2） were detected through the RT- PCR technology. Results Brushite stimulated the expression of osteogenesis markers and this action was further promoted by adding strontrium into brushite. This action was related to the concentration of strontrium. The effects on osteoblasts were most significant when the strontrium concentration was 0.5%. Conclusion Brushite has a very good biological activity and can play a favorable role in inducing the splitting and the differentiation of osteoblasts. And adding strontrium into brushite in different concentrations can promote the expression of VEGF, βFGF and Rurtx2 to certain degrees and thus significantly enhance their including effects on the differentiation of osteoblasts.

关 键 词：钛 锶 透钙磷石 成骨细胞 

分 类 号：R780.2[医药卫生—口腔医学]