TPX2在肝细胞癌组织中的表达及其临床意义  

Expression of TPX2 in hepatocellular carcinoma and its clinical significance

作　　者：梁博[1] 黄毓[2] 何华[1] 李嘉璐[1] 廖晖[1] 刘新城[1] 郭柏棠[1] 杨定华[1] 

机构地区：[1]南方医科大学南方医院肝胆外科,广州510515 [2]南方医科大学南方医院检验科,广州510515

出　　处：《中华医学杂志》2015年第6期408-411,共4页National Medical Journal of China

摘　　要：目的研究Xklp2靶蛋白（TPX2）在肝细胞癌患者癌组织中的表达水平及其与临床病理参数之间的关系。方法采用荧光定量PCR和免疫组化方法检测肝细胞癌患者癌组织及非癌组织中TPX2的表达水平，分析癌组织中TPX2的表达水平与临床病理参数之间的关系。结果TPX2mRNA在癌组织中的表达水平显著高于对应的非癌组织（30／50）（P〈0．05）；TPX2蛋白质在癌组织中的阳性率显著高于非癌组织（56．0％与16．0％，P〈0．05）。TPX2mRNA和蛋白质的表达水平与肿瘤发生转移、复发以及复发时间相关（P〈0．05）。结论TPX2高表达可能是肝细胞癌患者术后发生转移、复发的危险因素；TPX2可能是评价肝细胞癌预后的1个潜在生物学指标。Objective To explore the expression of TPX2 and its significance in hepatocellular carcinoma （HCC） tissue and examine the relationship between TPX2 and clinicopathological characteristics of HCC. Methods Reverse transcription-polymerase chain reaction （RT-PCR） and immunohistochemical staining were used to compare the expression of TPX2 in tumor and adjacent non-tumoral tissues. Results The TPX2 mRNA expression was higher in tumor tissues than that in non-tumoral counterparts （30/50） （ P 〈 0. 05 ）. The positive rate of TPX2 protein in tumor tissues was significantly higher than that in nontumoral counterparts （56. 0% vs 16. 0% ） （ P 〈 0. 05 ）. The expression of TPX2 mRNA and protein were correlated with metastasis, recurrence and time of recurrence after curative resection （ P 〈 0. 05 ）. Conclusion An over-expression of TPX2 may be risk factor of metastasis and recurrence after curative resection so that it is a potential biomarker for early diagnosis and prognosis of HCC.

关 键 词：癌 肝细胞 Xklp2靶蛋白 人 复发 肿瘤转移 

分 类 号：R735.7[医药卫生—肿瘤]