核素标记IL-11分子探针的构建及MHCC97H肝癌显像的研究  

Building a molecular probe of nine cyclic peptide and imaging in MHCC97H cells in vivo

作　　者：周欢[1] 吴清华[2] 陈道桢[3] 李怡佳[1] 孙晋[4] 张俊[4] 王强[4] 谢彦[4] 钟英[4] 刘璐[4] 周石[1] 

机构地区：[1]贵阳医学院附属医院介入科,贵州贵阳550004 [2]无锡市第三人民医院影像中心,江苏无锡214041 [3]南京医科大学附属无锡妇幼保健院,江苏无锡214002 [4]东南大学附属中大医院核医学科,江苏南京210009

出　　处：《东南大学学报（医学版）》2015年第1期32-36,共5页Journal of Southeast University(Medical Science Edition)

基　　金：国家自然科学基金资助项目(81372480;81202032);无锡市医院管理中心医学技术联合攻关项目(YGZX1213)

摘　　要：目的:构建核素标记自主合成九环肽DTPA-c(CGRRAGGSC)的生物分子靶向探针,研究其在肝癌体内分布及细胞定位。方法:Western blot检测人肝癌细胞MHCC97H及人正常肝细胞7702白介素11受体(IL-11R)表达情况;进行标记探针与MHCC97H细胞荧光定位;确定99Tcm标记DTPA-c(CGRRAGGSC)最佳条件;观察标记探针99Tcm-DTPA-c(CGRRAGGSC)在MHCC97H肝癌模型鼠体内生物分布。结果:IL-11R表达量MHCC97H细胞是7702细胞的21倍;荧光实验证实DTPA-c(CGRRAGGSC)与MHCC97H细胞膜和胞浆特异性结合;99Tcm-DTPA-c(CGRRAGGSC)标记率>98%;瘤体给药后进行ECT显像,证实具有良好靶向性及稳定性。结论:核素及荧光标记九环肽可靶向特异性结合肝癌MHCC97H肿瘤模型。Objective： To built a nuclide biological target probe and assess the biostribution in subcutaneous transplanted model of MHCC97H in nude mice. Methods：The expression changes of IL-11R in MHCC97H tumors and 7702 nomal hepatic cell were examined by Western blot. The binding site of the molecular target and internalization with MHCC97H cells was observed under a fluorescence microscope. DTPA-c（ CGRRAGGSC） was radiolabeled with 99Tcm. The biodisrtibution in nude mice bearing MHCC97H tumors after injection of 99Tcm-DTPA-c（ CGRRAGGSC） molecular probe was obsored. Results： Western blot showed the expression of IL-11R in MHCC97H cells was 21 times than that of 7702 cells. The c （ CGRRAGGSC） possessed the ability of specific binding with cytomembrane of MHCC97H cells. The labeling rate of 99Tcm-DTPA-c（CGRRAGGSC） was more than 98%. ECT imags confirmed good targeting and stability of 99Tcm-DTPA-c（CGRRAGGSC） probe in nude mice bearing MHCC97H. Conclusion：Nuclide marked DTPA-c（CGRRAGGSC） has specific binding to the MHCC97H tumor model of liver cancer.

关 键 词：九环肽 靶向探针 肝癌 核素 

分 类 号：R735.7[医药卫生—肿瘤] R817[医药卫生—临床医学]