遗传性癫痫大鼠海马内电压门控性钠离子通道四种亚型的表达及分布  

The expression and distribution of Na_v1.1,Na_v1.2,Na_v1.3 and Na_v1.6 in tremor rat hippocampus

作　　者：徐晓雪[1] 郭凤[2] 王千慧[2] 张朝东[1] 吕昕瞳 熊爽 王宏[1] 徐承伟[1] 赵晶[1] 蔡际群[2] 

机构地区：[1]中国医科大学附属第一医院神经内科神经病学研究所,沈阳110001 [2]中国医科大学药物毒理教研室,沈阳110122 [3]辽阳市第三人民医院,辽宁辽阳111000 [4]辽宁省分析科学研究院,辽宁沈阳110015

出　　处：《解剖科学进展》2015年第1期59-62,67,共5页Progress of Anatomical Sciences

基　　金：国家自然科学基金青年科学基金资助项目(No.81001429)

摘　　要：目的探讨遗传性癫痫大鼠(Tremor rat,TRM)大脑海马中,电压门控性钠离子通道Ⅰ、Ⅱ、Ⅲ、Ⅳ亚型(Na_v1.1、Na_v1.2、Na_v1.3与Na_v1.6)的表达及分布。方法 Western blot检测TRM(n=7)和Wistar大鼠(n=7)海马中Na_v1.1、Na_v1.2、Na_v1.3与Na_v1.6的蛋白表达情况。免疫荧光法进一步分析TRM及Wistar大鼠海马CAI、CA3和DG区中这四种亚型的分布与定位。结果相较于正常大鼠,TRM海马中Na_v1.1、Na_v1.2、Na_v1.3与Na_v1.6蛋白表达均明显上调(P<0.01)。此外,这四种VGSC亚型在TRM海马CAI,CA3区神经元及DG区颗粒细胞中分布广泛,且主要定位在细胞膜上。结论 Na_v1.1、Na_v1.2、Na_v1.3与Na_v1.6在TRM大鼠海马中出现显著高表达,可能与遗传性癫痫发生机制有关。Objective To explore the expression and distribution of voltage-gated sodium channel subtype Ⅰ, Ⅱ,Ⅲ, Ⅳ （Nav1.1, Nav1.2, Nav1.3 and Nav1.6） in tremor rat（TRM） hippoeampus. Methods The expressions of Nav1.1, Nay1.2, Nav1.3 and Nav1.6 were detected by Western blot, and the distributions of these proteins were analyzed by single-labeling immunofluorescence. Results Increased expression of Nav1.1, Nav1.2, Nav1.3 and Nav1.6 was observed in the hippocampus of TRM compared with normal rats. These four VGSC subtypes were found to be present throughout the hippocampal CA1, CA3 and dentate gyrus, especially localized on the membranes of pyramidal cells and granule cells. Conclusion The upregulated expressions of Nav1.1, Nav1.2, Nav1.3 and Nav1.6 may be involved in the pathogenesis of TRM epileptiform activity.

关 键 词：电压门控性钠离子通道 海马 遗传性癫痫大鼠 

分 类 号：R742.1[医药卫生—神经病学与精神病学]