大黄素对口腔鳞癌细胞Tca8113抑制作用  

Inhibitory effect of Emodin on oral squamous carcinoma cell line Tca8113

作　　者：豆颖[1] 闫哲[1] 潘翔珍[1] 刘秀芹[1] 

机构地区：[1]商丘市第一人民医院病理科,河南商丘476100

出　　处：《医药论坛杂志》2015年第1期72-74,共3页Journal of Medical Forum

摘　　要：目的观察大黄素对口腔鳞癌Tca8113细胞的抑制用研究。方法用20μmol/ml,40μmol/ml,60μmol/ml,80μmol/ml,100μmol/ml浓度的大黄素作用于口腔鳞癌Tca8113细胞48h,倒置显微镜下观察细胞的增殖情况,电镜下观察细胞的形态变化,并判定凋亡细胞。荧光染色进一步观察细胞的凋亡情况。结果倒置显微镜下观察到,随着大黄素浓度的升高,细胞的增殖能力随之下降,电镜观察到凋亡细胞,AO/EB染色亦观察到,随着大黄素浓度的增高,处于晚期凋亡的细胞随之增多。结论大黄素可抑制口腔鳞癌Tca8113细胞的增殖。Objective The inhibition and the effect of emodin on Tea8113 cell line. Methods Human oral squamous carcinoma Tca8113 cells are randomized into control 20mol/1,40mol/1,60mol/1,80mol/l, 100mol/l groups respectively cultivate for 48h,Inverted microscope were used to observed cells proliferation, transmission electron were used to observe cell uhrastructure changes and determine the apoptotic cells. Fluorescent dye further observation of cell apoptosis. Results Tca8113 cell line proliferation ability drops with the increase of concentration of emodin, transmission electron and AO/EB immunofluorescence staning results show with the increase of concentration of emodin, the more cells in the late apoptosis. Conclusion Emodin can effectively inhibit the proliferation of Tea8113 cells.

关 键 词：大黄素 口腔鳞癌细胞Tca8113 抑制 

分 类 号：R739.8[医药卫生—肿瘤]