孝感地区7780例TCT与HPV联合检测分析  

7780 cases TCT combined with HPV testing correlation analysis in Xiaogan

作　　者：何惠华[1] 刘汉忠[1] 李蓓[1] 杨继洲[1] 

机构地区：[1]华中科技大学同济医院附属孝感市中心医院病理科,湖北孝感432000

出　　处：《临床与病理杂志》2015年第2期258-262,共5页Journal of Clinical and Pathological Research

摘　　要：目的：探讨孝感地区妇女不同年龄阶段、不同TCT结果中人乳头瘤病毒（human papillomavirus,HPV）感染特点。方法：对2010年1月-2013年12月在孝感市中心医院体检及门诊就诊的7 780例宫颈细胞标本进行液基薄层细胞学（liquid-based thinprep cytology test,TCT）检测及采用表面等离子体谐振（SPR）技术,进行HPV基因分型检测。结果：24型HPV均被检出,阳性率为20.55%,感染以高危型HPV（hrHPV）为主;HrHPV阳性率为18.07%,其中HPV16型阳性最多;7 075例上皮内病变及恶性肿瘤为阴性（negative for intraepithelial lesions and malignancies,NILM）中hrHPV阳性率为12.28%,但NILM与其他各组的病毒平均负载量统计学有差异。随着病毒平均负载量的增大,TCT结果显示宫颈病变程度越重。HrHPV感染者中26岁以下组感染率最高。结论：孝感地区HPV感染状况具有地域性特点。TCT结果、HPV感染状况及其病毒负载量以及年龄因素的综合分析对指导临床制定下一步诊疗方案具有重要意义。Objective： To study the human papillomavirus（HPV） genotypes and their distribution character in uterine cervix in Xiaogan. Methods： The investigated group comprised 7 780 patients from Xiaogan Central hospital since Jan 2010 to Oct 2013. All the cervical samples were analyzed by TCT and the SPR biosensor. The SPR biosensor could detect 16 types of high-risk HPV and 8 types of low-risk HPV at the same time. Results： Twenty four HPV genotypes were detected out, the positive rate was 20.55%, the infection based on hrHPV; the positive rate of hrHPV was 18.07%, among which the most HPV16 positive. HrHPV positive rate was 12.28% in 7 075 cases NILM, but there was a statistical difference in the average virus load of NILM from other groups. With an average viral load increasing, TCT showed more severe cervical lesions. hrHPV infected persons in 26 years of age group has the highest infection rate. Conclusion： HPV infection has regional characteristics in Xiaogan.A comprehensive analysis of TCT results, HPV infection status, viral load and the age factor in guiding clinical treatment programs was of significance.

关 键 词：人乳头状瘤病毒 宫颈上皮内瘤病变 表面等离子体谐振(SPR) 病毒负载量 

分 类 号：R737.33[医药卫生—肿瘤]