GSK-3β在雷公藤甲素诱导胰腺癌细胞凋亡中的作用  

Role of GSK-3β in triptolide induced apoptosis of pancreatic cancer cells

作　　者：毛振江[1] 李国庆[1] 黄靓[1] 

机构地区：[1]南华大学附属第二医院消化内科,湖南省衡阳市421001

出　　处：《世界华人消化杂志》2015年第2期256-260,共5页World Chinese Journal of Digestology

摘　　要：目的:分析糖原合酶激酶3β(glycogen synthase kinase 3β,GSK-3β)在雷公藤甲素(triptolide TPL)诱导胰腺癌细胞As PC-1凋亡中的作用.方法:体外培养胰腺癌细胞As PC-1,采用TPL处理As PC-1细胞及采用GSK-3β抑制剂Li C预处理As PC-1,流式细胞仪分析细胞凋亡情况及采用Western blot分析GSK-3β、p-糖原合酶激酶-3β(p-glycogen synthase kinase3β,p-G S K-3β)及B细胞淋巴瘤-2(B-c e l lymphoma-2,Bcl-2)等蛋白表达水平.结果:6.54 ng/m L及15.51 ng/m L TPL处理对A s P C-1细胞活性有显著的抑制作用,分别为39.64%及52.19%,经L i C l预处理后其抑制作用减弱为27.36%及41.94%;采用Li Cl预处理组的细胞凋亡情况较相应浓度未经Li Cl预处理组As PC-1细胞的凋亡率降低;经TPL处理后As PC-1细胞中p-GSK-3β水平显著增加,而GSK-3β的表达水平无显著性变化;采用Li Cl预处理后As PC-1细胞中p-GSK-3β的表达水平增加,GSK-3β的表达水平无显著性变化,且凋亡相关因子Bcl-2、B细胞淋巴瘤-xl(B-cell lymphoma-xl,Bcl-xl)及髓样细胞白血病-1(myeloid cell leukemia-1,Mcl-1)的表达水平降低.结论:p-GSK-3β水平增加能够抑制TPL诱导的胰腺癌细胞的凋亡.AIM： To investigate the role of glycogen synthase kinase 3 β（ GSK- 3β） in triptolide induced apoptosis of pancreatic cancer cells. METHODS： Pancreatic cancer As PC-1 cells were treated with triptolide in the presence or absence of GSK-3β inhibitor Li Cl. Cell apoptosis was assessed using flow cytometry. The expression of GSK-3β, p-glycogen synthase kinase-3β（p-GSK-3β） and B-cell lymphoma-2（Bcl-2） proteins was detected by Western blot. RESULTS： Treatment with triptolide（TPL） at 6.54 ng/m L and 15.51 ng/m L significantly reduced the growth of AsP C-1 cells, and the rates of reduced growth were 39.64% and 52.19%,respectively. Li Cl pretreatment reduced therates of reduced growth to 27.36% and 41.94%, respectively. Li Cl pretreatment significantly reduced the apoptosis rate of As PC-1 cells. Triptolide treatment significantly increased the level of p-GSK-3β in As PC-1 cells, but had no significant impact on GSK-3β expression; Li Cl pretreatment significantly increased the expression level of p-GSK-3β in AsP C-1 cells, had no significant impact on GSK-3β expression, and significantly reduced the expression of apoptosisrelated factors Bcl-2, B-cell lymphoma-xl（Bcl-xl） and myeloid cell leukemia-1（Mcl-1）. CONCLUSION： The increased level of p-GSK-3β can inhibit triptolide induced pancreatic cancer cell apoptosis.

关 键 词：糖原合酶激酶3 雷公藤甲素 胰腺癌 细胞凋亡 

分 类 号：R735.9[医药卫生—肿瘤]