乳腺癌原发灶成纤维细胞活化蛋白α表达与患者预后相关  

The expression of fibroblast activation protein-α in primary breast cancer is associated with poor prognosis

作　　者：于海明[1] 杨俊兰[1] 李莹[1] 焦顺昌[1] 

机构地区：[1]解放军总医院肿瘤内科,北京100853

出　　处：《细胞与分子免疫学杂志》2015年第3期370-374,共5页Chinese Journal of Cellular and Molecular Immunology

基　　金：吴阶平医学基金(320.6752.1230)

摘　　要：目的本研究旨在探讨乳腺癌原发灶成纤维细胞活化蛋白α(FAP-α)表达与临床病理特征以及预后的关系。方法回顾性收集2000-01-01/2002-12-31期间在解放军总医院手术的130例Ⅰ-Ⅲ期乳腺癌患者的临床资料及石蜡切片,免疫组织化学染色检测乳腺癌原发灶FAP-α表达,分析FAP-α表达与转化生长因子β1(TGF-β1)表达强度的关系,并分析FAP-α表达与乳腺癌预后的关联性以及与临床病理特征的相关性。结果 FAP-α表达在肿瘤细胞和间质成纤维细胞的细胞质,FAP-α阳性纤维细胞密度与TGF-β1阳性间质细胞密度正相关。FAP-α肿瘤细胞质强度与TGF-β1阳性肿瘤细胞质表达正相关。在雌激素受体和孕激素受体均阴性患者中,FAP-α阳性纤维细胞密度是无病生存(DFS)和总生存(OS)的独立不良预后因素;FAP-α阳性肿瘤细胞质强度是DFS和OS的独立不良预后因素。结论激素受体阴性乳腺癌患者中,原发灶FAP-α高表达与不良预后相关。乳腺癌原发灶TGF-β1的表达与FAP-α的表达正相关。Objective To evaluate the prognostic value of fibroblast activation protein-α （FAP-α） expression in primary lesions of breast cancer patients and the relationship with the clinicopathologic features. Methods Paraffin sections and clinical data were retrospectively collected from 130 stage Ⅰ-Ⅲ breast cancer patients who had underwent surgery at the Chinese PLA General Hospital from January 1st, 2000 to December 31st, 2002, Immunohistochemistry was used to assess FAP-α expression in primary lesions of breast cancer to evaluate the associations of FAP-cc expression with transforming growth factor-β1（TGF-β1） expression in breast cancer and patients＇ clinicopathologic characteristics and prognosis. Results FAP-q was seen in cytoplasm of tumor cells and interstitial fibroblasts. Density of interstitial FAP-α-positive fibroblasts was positively correlated with the staining density of interstitial TGF-β-positive cells. Intensity of CTLA-4 expression in tumor cells was positively correlated with TGF-β1 expression in tumor cells. In patients whose lesions were negative for both estrogen and progesterone receptors, density of interstitial FAP-a-positive fibroblasts was an independent adverse prognostic factor for disease-free survival （DFS） and overall survival （OS） ; intensity of CTLA-4 expression in tumor cells was an independent adverse prognostic factor for DFS and OS. Conclusion High expression of FAP-α in primary breast tumors is associated with a bad prognosis in patients whose lesions are negative for both estrogen and progesterone receptors. Expression of TGF-β1 is positively related with FAP-α expression in primary breast tumors.

关 键 词：成纤维细胞活化蛋白α 转化生长因子-Β1 乳腺癌 预后 

分 类 号：R737.9[医药卫生—肿瘤] R392-33[医药卫生—临床医学]