油菜品种及高油育种材料遗传多样性SSR分析  

Genetic Diversity of Rapeseed Varieties and High-oil Inbred Lines Using SSR Markers

作　　者：李晓荣[1] 张菁雯[1] 赵燕妮[1] 徐献锋 胡玉梅[1] 于澄宇[1] 

机构地区：[1]西北农林科技大学农学院,陕西杨陵712100

出　　处：《西北植物学报》2015年第2期258-268,共11页Acta Botanica Boreali-Occidentalia Sinica

基　　金：教育部新世纪优秀人才(NECT-10-0693);中央高校基本科研业务费(QN2013084)

摘　　要：通过SSR标记分析91份油菜品种、骨干亲本和高油自交系,以及作为参考物种的芥菜型油菜、白菜型油菜、埃塞俄比亚芥等8份材料之间的亲缘关系及遗传多样性,为油菜杂种优势利用育种提供理论依据。筛选48对SSR引物对99份参试材料进行扩增,共检测到255个多态性条带。UPGMA聚类将99份材料聚为5组:第Ⅰ组包括89个材料,均为甘蓝型油菜;第Ⅱ组有埃塞俄比亚芥和‘G8白’2个白花材料;第Ⅲ组包括4个芥菜型材料;第Ⅳ组包括3个白菜型材料;第Ⅴ组仅有‘秦芥2008’。第Ⅰ组89个甘蓝型油菜又可分为5个亚组。第i亚组包括43个材料,主要为陕西的恢复系、华中农业大学和浙江材料;第ii亚组包括9个材料,以陕西材料为主;第iii亚组包括12个材料,以贵州和国外材料为主;第iv亚组包括19个材料,主要为中国农科院油料作物所、浙江农科院和安徽的材料;第v亚组包括‘浙油758’、‘147C’等6个材料。而且来自同一育种单位的大部分材料具有较高相似性,这可能是由于育种单位频繁使用少数核心骨干亲本所造成的。In this study,genetic relationship and diversity based on SSR markers were investigated in 91 rapeseed accessions including registered varieties,backbone parents,and high-oil inbred lines,as well as 8 references from Brassica juncea,B.campestris and B.carinata.48 pairs of selected SSR primers from A and C genomes were employed and 255 polymorphic bands were detected.UPGMA clustering showed that 99 accessions were divided into 5 groups.The largest group,group Ⅰ included 89 B.napus materials.Group Ⅱ only included 2 white flower accessions named Ethiopia mustard and G8.Group Ⅲ consisted of 4 B.juncea landraces.Group Ⅳ were 3 B.campestris landraces and group Ⅴ contained only a mustard named Qinjie 2008,respectively.The 89 B.napus in group Ⅰ could be further divided into 5 subgroups.Subgroup 1 included 43 materials,most of which were restorer lines from Shaanxi and materials from Huazhong Agricultural University and Zhejiang.Subgroup 2 had 9 materials mainly from Shaanxi.12 materials were included in subgroup 3 and most of them were Guizhou and foreign materials.Subgroup 4 consisted of 19 materials mainly from Oil Crops Research Institute,CAAS,Zhejiang Academy of Agricultural Sciences,and Anhui.Subgroup 5 included 6 materials,such as Zheyou 758 and 147C.Most materials from same institutions had a higher similarity.The reason might be that these varieties or lines were bred with limited core inbred lines.The results could offer helpful information for rapeseed breeding and utilization of heterosis.

关 键 词：油菜 遗传多样性 SSR 含油量 

分 类 号：Q789[生物学—分子生物学]