水稻香味基因分子标记的开发及应用  

Development of Molecular Markers for Fragrant Gene and Its Application

作　　者：闫影[1] 诸光明 张丽霞[1] 万常照[1] 曹黎明[1] 赵志鹏[1] 吴书俊[1] 

机构地区：[1]上海市农业科学院作物育种栽培研究所,上海201403 [2]上海市嘉定区农业技术推广服务中心,上海201800

出　　处：《西北植物学报》2015年第2期269-274,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：上海市农业科学院青年基金[农青年科技2013(17)];上海市现代水稻产业技术体系建设[沪农科产字(2014)第3号]

摘　　要：根据香稻材料在Badh2基因(甜菜碱醛脱氢酶基因)第7外显子的突变位点,设计了分子标记YY5-YY8用来区分这种突变类型的香稻材料。同时结合已报道的针对Badh2在第2、4、5外显子处突变设计的功能标记InDel-E2和FMbadh2-E4-5,对80份不同生态区香稻材料进行了标记检测。结果表明,26份材料属于第7外显子突变类型,37份材料属于第2外显子突变类型,无第4、5外显子突变类型材料。该研究构建了针对Badh2突变的新的分子标记YY5-YY8,并且鉴定了上述香稻材料的突变类型,为优质香稻分子育种奠定基础。According to the mutation in exon 7 of Badh2 （Betaine aldehyde dehydrogenasegene） in fragrant rice,we designed a functional marker YY5-YY8,which can be used to check rice materials with this mutation or not.Other two reported markers for exon 2-mutated and exon 4 or 5-mutated types of Badh2,InDel-E2 and FMbadh2-E4-5,were also used to analyze the mutations of Badh2 in 80 fragrant rice materials from different ecological regions.It showed that 26 fragrant rice materials were of the exon 7-mutated type,37 fragrant rice materials were of exon 2-mutated type and none of them was found to be of exon 4 or 5-mutated type.This study constructed a new molecular marker YY5-YY8 for checking the mutation of Badh2 and identified the mutation types of these fragrant rice varieties for breeding high quality fragrant rice.

关 键 词：水稻 香味基因 分子标记 

分 类 号：Q789[生物学—分子生物学]