软枣猕猴桃叶片光系统Ⅱ活性对不同温度的响应  

Activity of Photosystems Ⅱ in Leaves of Actinidia arguta under Different Temperature Treatments

作　　者：王振兴[1] 艾军[1] 陈丽[2] 范书田[1] 何伟[1] 秦红艳[1] 赵滢[1] 

机构地区：[1]中国农业科学院特产研究所,长春130112 [2]吉林农业大学园艺学院,长春130112

出　　处：《西北植物学报》2015年第2期329-334,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：吉林省科技发展计划(20130102046JC);吉林省科技发展计划(20140204034NY)

摘　　要：以软枣猕猴桃“魁绿”为试验材料,利用快速叶绿素荧光诱导动力学曲线分析技术（JIP-test）研究了热胁迫处理对植株叶片光系统Ⅱ活性的影响。结果显示：（1）软枣猕猴桃叶片最大光化学效率（Fv/Fm）在35～48 ℃的范围内并没有明显变化,只有当温度升高到52 ℃时才显著下降。（2）随着温度升高,叶绿素荧光诱导动力学曲线中J点和I点的相对可变荧光Vj和Vi呈显著下降趋势,在52 ℃又显著升高,而K点的相对可变荧光Vk则逐渐上升;叶片捕获的激子将电子传递到电子传递链中QA-下游电子受体的概率（ψ0）随着温度升高而逐渐上升,但在52 ℃时显著下降。（3）随着热胁迫时间的延长,Vj和Vi随时间延长而升高,ψ0则下降,电子传递链受体侧受到了严重的抑制。研究表明,高温显著抑制了软枣猕猴桃叶片PSⅡ电子传递链供体侧和受体侧的活性,但PSⅡ的供体侧比受体侧对高温更加敏感;JIP-test测定的相关参数能有效地评价不同温度对软枣猕猴桃光系统活性的影响。The effects of heat stress on activities of photosystem Ⅱ in leaves of Actinidia arguta （Sieb.et Zucc.） Planch.ex Miq.（Kuilü,a cultivar） were studied by chlorophyll a fluorescence transient and JIP-test.The results showed that：（1）Fv/Fm of leaves did not significantly change at 35-48 ℃,but decreased at 52 ℃.（2）With increase of leaf temperature,the relative variable fluorescence at J and I steps of fluorescence transient （Vj and Vi） significantly decreased,but increased at 52 ℃,the relative variable fluorescence Vk increased gradually.（3）With the increase of heat treatment time,the Vj and Vi increased gradually,but Ψo decreased gradually,indicating that the acceptor side of PSⅡ was damaged severely.In summary,activity of the donor side and the acceptor side of PSⅡ was inhibited by high temperature.The donor side was more sensitive to heat stress.JIP-test parameters can evaluate effectively activity of photosystems Ⅱ in leaves of A.arguta under different temperatures.

关 键 词：软枣猕猴桃 高温胁迫 光系统 JIP-TEST 

分 类 号：Q945.79[生物学—植物学]