CagA和IL-9在幽门螺杆菌感染伴消化性溃疡和胃息肉患者中的表达及其作用研究  

Expressions and Effects of CagA and IL-9 in Helicobacter pylori Infected Patients with Peptic Ulcer and Gastric Polyps

作　　者：陈周利 

机构地区：[1]广东省惠州市惠东县人民医院内二科,516300

出　　处：《胃肠病学》2015年第2期81-84,共4页Chinese Journal of Gastroenterology

摘　　要：背景:研究表明幽门螺杆菌(Hp)感染与消化性溃疡(PU)和胃息肉(GP)的发生相关。细胞毒素相关蛋白A(CagA)是Hp致病的重要毒力因子,而白细胞介素-9(IL-9)是具有抗炎效应的细胞因子。目的:探讨CagA、IL-9在Hp感染伴PU和GP患者中的表达及其作用。方法:纳入2013年1月-2014年3月于惠东县人民医院就诊的Hp感染伴PU(Hp+PU组)或Hp感染伴PU和GP患者(Hp+PU+GP组),无Hp感染的慢性胃炎患者作为对照组。采用ELISA法检测血清CagA、IL-9水平;采用免疫组化法检测胃黏膜组织CagA、IL-9表达。结果:Hp+PU组、Hp+PU+GP组血清CagA水平均显著高于对照组(P<0.01),血清IL-9水平均显著低于对照组(P<0.01),且Hp+PU+GP组血清IL-9水平显著低于Hp+PU组(P<0.05)。Hp+PU组、Hp+PU+GP组CagA+患者比例均显著高于对照组(P<0.05);三组患者中,CagA+患者血清IL-9水平均显著低于CagA-患者(P<0.01),Hp+PU+GP组CagA+患者血清IL-9水平显著低于Hp+PU组和对照组CagA+患者(P<0.05)。Hp+PU组、Hp+PU+GP组胃黏膜CagA阳性面积比值显著高于对照组(P<0.01),Hp+PU+GP组又显著高于Hp+PU组(P<0.05)。Hp+PU组、Hp+PU+GP组胃黏膜IL-9阳性面积比值显著低于对照组(P<0.05),Hp+PU+GP组又显著低于Hp+PU组(P<0.01)。结论:CagA+Hp感染可能与PU和GP的发生有关。IL-9参与了Hp感染诱导的免疫应答,且可能对Hp感染所致的PU和GP具有抑制作用。Background： Studies showed that Helicobacter pylori（ Hp） infection was closely related with the pathogenesis of peptic ulcer（ PU） and gastric polyps（ GP）. Cytotoxin-associated protein A（ CagA） is an important virulence factor of Hp,and interleukin-9（ IL-9） is an anti-inflammatory cytokine. Aims： To investigate the expressions and effects of CagA and IL-9 in Hp infected patients with PU and GP. Methods： Hp infected patients with PU or PU ＋ GP from January 2013 to March 2014 at Huidong County People＇s Hospital were enrolled,and categorized into Hp ＋ PU group and Hp ＋ PU ＋ GP group. Patients with chronic gastritis without Hp infection were served as control group. Serum levels of CagA and IL-9were measured by ELISA method. Expressions of CagA and IL-9 in gastric mucosal tissue were determined by immunohistochemisty. Results： In Hp ＋ PU group and Hp ＋ PU ＋ GP group,serum levels of CagA were significantly higher and serum levels of IL-9 were significantly lower than those in control group（ P 0. 01）,and serum level of IL-9 in Hp ＋PU ＋ GP group was significantly lower than that in Hp ＋ PU group（ P 0. 05）. Proportions of patients with CagA ＋in Hp ＋PU group and Hp ＋ PU ＋ GP group were significantly higher than that in control group（ P 0. 05）. Serum levels of IL-9 in patients with CagA ＋were significantly lower than those in patients with CagA-in all three groups（ P 0. 01）. Serum levels of IL-9 in patients with CagA ＋in Hp ＋ PU ＋ GP group were significantly lower than those in patients with CagA ＋in Hp ＋ PU group and control group（ P 0. 05）. Positive area ratio of CagA in gastric mucosal tissue in Hp ＋ PU group and Hp ＋ PU ＋ GP group were significantly higher than that in control group（ P 0. 01）,and that in Hp ＋ PU ＋ GP group was significantly higher than that in Hp ＋ PU group（ P 0. 05）. Positive area ratio of IL-9 in gastric mucosal tissue in Hp ＋ PUgroup and Hp ＋ PU ＋ GP group were significantly lower than

关 键 词：幽门螺杆菌 消化性溃疡 胃息肉 细胞毒素类 白细胞介素9 

分 类 号：R573.1[医药卫生—消化系统]