MicroRNA-137抑制胰腺癌细胞侵袭和迁移能力  

MicroRNA-137 Inhibits Human Pancreatic Cancer Cell Invasion and Migration

作　　者：陈美源 肖杰[1] 江建新[1] 喻超[1] 张宏[1] 陈玲[1] 孙诚谊[1] 

机构地区：[1]贵阳医学院附院肝胆外科,贵州贵阳550004

出　　处：《贵阳医学院学报》2015年第2期112-116,120,共6页Journal of Guiyang Medical College

基　　金：国家国际科技合作专项资助(2014DFA31420);国家自然科学基金资助项目(81160311);贵州省科技厅项目[黔科合(2010)3149];贵州省科技厅联合基金计划项目[黔科合LH字(2014)7129]

摘　　要：目的:探讨microRNA-137(miR-137)对胰腺癌细胞侵袭和迁移能力的影响。方法:构建miR-137慢病毒表达载体(LV-miR-137)及空载体,将细胞分为3组:LV-miR-137感染组(实验组)、空载体组(阴性对照组)及空白组;LV-miR-137感染胰腺癌PANC-1和MIAPaCa2细胞后72h,实时荧光定量PCR(qRT-PCR)检测感染效率,Transwell小室侵袭实验及细胞划痕实验检测miR-137对PANC-1和MIAPaCa2细胞株侵袭和迁移能力的影响。结果:LV-miR-137感染胰腺癌细胞株PANC-1和MIAPaCa2后miR-137表达水平量明显升高,Transwell小室细胞侵袭实验发现:实验组PANC-1和MIAPaCa2细胞迁移能力明显受到抑制,细胞迁移数目(58.2±1.1,37.5±1.8)较空白对照组(141.7±7.9,74.2±2.4)和阴性对照组(151.7±5.8,72.2±2.9)明显减少,P<0.05,差异有统计学意义;PANC-1细胞的侵袭能力也明显受到抑制,细胞侵袭数目(44.0±1.5)较空白对照组(110.9±6.4)和阴性对照组(117.2±1.9)明显减少,P<0.05,差异有统计学意义;细胞划痕实验检测发现,24h后实验组胰腺癌MIAPaCa-2、PANC-1细胞的迁移距离明显比阴性对照组及空白组短,P<0.05,差异有统计学意义。结论:MiR-137能抑制胰腺癌细胞的侵袭和迁移能力,有望成为胰腺癌基因治疗的新靶点。Objective： To investigate the effect of miR-137 on invasion and metastasis of human pan- creatic cancer cells. Methods： miR-137 lentiviral vector （LV-miR-137） and the empty vector were constructed, the cells were divided into three groups： LV-miR-137 infected group （ experimental group ） , empty vector （ negative control group ） and control group. After PANC-1 and MIA PaCa2 cells were infected with LV-miR-137 for 72 h, real time quantitative PCR （qRT-PCR） was applied to detect the infection efficiency. The Transwell chamber assay and cell wound healing were employed to observe the effects of LV-miR-137 on invasion and metastasis of MIA PaCa2 and PANC-1 cell lines. Results： After infected with LV-miR-137, the miR-137 expression levels in MIA PaCa2 and PANC-1 cells increased. The Transwell chamber assay found that the migration ability of MIA PaCa2 and PANC- 1 ceils in experimental group was significantly inhibited, the cell migration number （37.5 ± 1.8, 58.2 ±1.1 ） was obviously lower than that of control group （74.2 ± 2.41,141.7 ± 7.9） and negative con- trol group （72.2 ±2.91,151.7 ±5.8）, P 〈0.05. PANC-1 cell invasion ability was also significantly inhibited, the number of cell invasion （44.0 ± 1.5 ） was lower than that of control group （ 110.9 ±6. 4） and negative control group （ 117.2 ± 1.9 ） , P 〈 0.05. Wound-healing assay showed that MIA PaCa-2, PANC-1 cell migration distance was shorter than that of negative control group and control group after 24 h experiment, P 〈 0.05. Conclusion： MiR-137 can inhibit pancreatic cancer cell mi- gration and invasion, which may be a new target for gene therapy of pancreatic cancer.

关 键 词：胰腺肿瘤 侵袭 迁移 微小RNA 

分 类 号：R735.9[医药卫生—肿瘤]