MicroRNA-137在胰腺癌中的表达及临床意义  

The Expression of MicroRNA-137 in Pancreatic Cancer and Its Clinical Significance

作　　者：喻超[1] 肖杰[1] 江建新[1] 潘耀振[1] 黎志鹏[1] 张宏[1] 孙诚谊[1] 

机构地区：[1]贵阳医学院附院肝胆外科,贵州贵阳550004

出　　处：《贵阳医学院学报》2015年第2期117-120,共4页Journal of Guiyang Medical College

基　　金：国家国际科技合作专项资助(2014DFA31420);国家自然科学基金资助项目(81160311);贵州省科技厅项目[黔科合(2010)3149];贵州省科技厅联合基金计划项目[黔科合LH字(2014)7129]

摘　　要：目的：检测MicroRNA-137（miR-137）在胰腺癌中的表达并探讨其临床意义。方法：采用实时荧光定量PCR（qRT-PCR）检测miR-137在17例临床胰腺癌组织及相配对的癌旁组织中的表达,同时,检测8株不同胰腺癌细胞系和2株正常胰腺上皮细胞中miR-137的表达,并分析miR-137的表达水平与胰腺癌临床病理参数之间的关系。结果：miR-137在胰腺癌组织中的表达水平明显低于癌旁组织,差异具有统计学意义（P〈0.05）;miR-137在胰腺癌细胞系中的表达水平明显低于正常胰腺上皮细胞（P〈0.05）;miR-137表达水平在临床Ⅰ~Ⅱ期胰腺癌组织明显高于Ⅲ~Ⅳ期胰腺癌组织（P〈0.05）,在无淋巴转移的胰腺癌组织明显高于有淋巴转移的胰腺癌组织（P〈0.05）。结论：miR-137低表达与胰腺癌的发生发展相关,可能成为胰腺癌基因诊断和治疗的新靶点。Objective： to investigate its clinical To detect microRNA-137 （miR-137） expression in pancreatic carcinoma, and significance. Methods： Real-time PCR （qRT-PCR） was used to detect miR- 137 expression in 17 cases of pancreatic carcinoma tissues and matched paracancerous tissues, 8 pan- creatic cancer cell lines and 2 normal pancreatic epithelial cell lines; the relationship between the ex- pression level of miR-137 in pancreatic cancer and pancreatic cancer clinical pathological parameters was analyzed. Results： Expression level of miR-137 in pancreatic cancer tissue was significantly lower than that of adjacent tissues （P 〈0.05 ）. Expression level of miR-137 in pancreatic cancer cell lines was significantly lower than that in normal cell lines （P 〈 0.05 ）. The expression level of miR-137 in the I -II stage pancreatic cancer tissue was significantly higher than that in m - rv stage pancreatic carcinoma （ P 〈 0.05 ）, that in pancreatic cancer without lymph node metastasis was significantly high- er than that of pancreatic cancer with lymph node metastasis （P 〈 0.05 ）. Conclusion： MiR-137 is re- lated to the occurrence and development of pancreatic cancer. It may be a new target of gene diagnosis and therapy of pancreatic cancer.

关 键 词：胰腺肿瘤 肿瘤分期 淋巴转移 微小RNA 

分 类 号：R735.9[医药卫生—肿瘤]