幽门螺杆菌对小鼠胃黏膜G、D细胞及胃泌素和生长抑素的影响  

Changes of Gastric Mucosal G,D Cells and m RNA Levels of Gastrin and Somatostatin in Helicobacter pylori-infected Mice

作　　者：张岚[1] 孙朝琴[1] 张姝[1] 张然[2] 莫非[1] 

机构地区：[1]贵阳医学院检验学院,贵州贵阳550004 [2]聊城市人民医院检验科,山东聊城252000

出　　处：《贵阳医学院学报》2015年第2期130-134,137,共6页Journal of Guiyang Medical College

基　　金：高等学校特色专业建设点[(2010)15];贵州省科技厅;贵阳医学院联合基金资助项目[黔科合LG(2012)054号];贵州省科技厅;贵阳医学院联合基金资助项目[黔科合LG(2012)012号];贵州省科技计划课题[黔科合J字(2014)2027号];贵阳医学院附院博士基金项目(2014)

摘　　要：目的:研究幽门螺杆菌(Hp)感染对胃黏膜G、D细胞及胃泌素(GAS)、生长抑素(SS)表达的影响。方法:Hp标准菌SS2000菌液灌胃C57BL/6小鼠构建Hp感染小鼠慢性胃炎动物模型,免疫组化染色观察小鼠胃黏膜G、D细胞的改变,图像分析软件(Image-Pro-Plus,IPP)分析GAS、SS阳性灰度值,Real time-PCR检测小鼠胃窦黏膜GAS mRNA和SS mRNA相对表达量。结果:与空白组比较,模型组G细胞数目、GAS灰度值、GAS m RNA表达水平显著增高(P<0.05),D细胞数目、SS灰度值、SS mRNA表达水平无明显改变。结论:Hp感染可引起胃黏膜GAS mRNA表达上调,胃窦黏膜G细胞增多,促进GAS分泌,可能是胃黏膜病理改变加重的原因。Objective： To investigate the influence of Helicobacter pylori （lip） infection on gastric mucosal G cells and D cells, and the expression of gastrin （GAS） and somatostatin （SS） mRNA in mice. Methods： SPF C57BL/6 mice were given Hp SS2000 by gavage to construct animal model of chronic gastritis. Adopting immunohistochemistry starting and image-pro-plus （IPP） to detect the ex- pression and localization of GAS and SS in gastric antrum tissue, and analyzing the changes on G cell and D cell. The GAS and SS mRNA expression levels in gastric mucosa were detected before and after Helicobacter pylori infected by real time PCR. Results： Comparing with the blank group, model group＇s number of G cells, GAS gray value, GAS expression level of mRNA was significantly increased （P 〈 0.05） ; the number of D cells, SS gray value, SS mRNA expression level of model group did not sig- nificant changed （ P 〉 0.05 ）. Conclusions： The infection of Hp can upregulate the expression of GAS mRNA in gastric mucosa, and increase the amount of G cells in gastric mucosa, and promote the se- cretion of GAS. It may be a cause of gastric mucosal pathological change aggravation.

关 键 词：螺杆菌 幽门 G细胞 D细胞 胃泌素 生长抑素 

分 类 号：R363.2[医药卫生—病理学] R573.3[医药卫生—基础医学]