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作 者:李健[1] 张颖[1] 马林[1] 张凯强[1] 雷双[1] 顾何锋[1]
机构地区:[1]中国医科大学附属口腔医院口腔预防科,辽宁省口腔医学研究所口腔预防医学教研室,中国医科大学附属口腔医院中心实验室,辽宁沈阳110002
出 处:《上海口腔医学》2015年第1期1-5,共5页Shanghai Journal of Stomatology
基 金:国家自然科学基金(81072245);辽宁省自然科学基金(20102278)~~
摘 要:目的:研究GRP-78和caspase-12在氟致大鼠成釉细胞内质网应激和细胞凋亡中的作用,探讨氟所介导的内质网应激是否导致细胞凋亡。方法:应用CCK8和流式细胞术观察不同浓度的氟对成釉细胞活性及凋亡数量的影响,以实时定量PCR和Western印迹法检测氟对内质网伴侣分子GRP-78和caspase-12基因和蛋白表达的影响,应用SPSS13.0软件包对数据进行统计学分析。结果:随着氟浓度的不断增加,大鼠成釉细胞活性呈逐渐下降趋势,流式细胞术同样显示发生凋亡的细胞数量逐渐上升;实时定量RT-PCR和Western印迹结果显示,GRP-78和caspase-12的表达量随着氟浓度增加而增加。结论:过量氟介导了大鼠成釉细胞内质网应激,并且通过内质网应激,引起细胞凋亡。PURPOSE: To study the effect of GRP-78 and caspase-12 on fluoride-induced endoplasmic reticulum stress and apoptosis in rat ameloblast, and explore whether fluoride-induced endoplasmic reticulum stress results in the occurrence of apoptosis. METHODS: The cell activity of ameloblast cultured in various concentrations of fluoride was measured by CCK8 and flow cytometry; Real-time RT-PCR and Western blot were used to analyze the endoplasmic reticulum chaperone GRP-78 and caspase-12 genes and the expression of related proteins. The data was analyzed with SPSS13.0 software package. RESULTS: With the increasing concentration of fluoride, the cell activity of rat ameloblasts decreased gradually,and flow cytometry also showed that the number of apoptosis gradually increased; Real-time RT-PCR and Western blot showed the expression of GRP-78 and caspase-12 increased while the fluoride concentration increased. CONCLUSIONS:Excessive fluoride induces endoplasmic reticulum stress of rat ameloblast, and leads to cell apoptosis.
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